结核与肺部疾病杂志2025，Vol.6Issue(4)：420-425,6.DOI:10.19983/j.issn.2096-8493.20250062
杭州郊县区非结核分枝杆菌肺病病原菌鉴定及其耐药性分析
Identification and drug resistance analysis of non-tuberculous mycobacterial pulmonary disease pathogens in suburban areas of Hangzhou
摘要
Abstract
Objective:To understand the prevalence and drug resistance of non-tuberculous mycobacteria(NTM)caused lung disease pathogens in suburban counties in Hangzhou in recent years.Methods:PNB/TCH growth tests were used to preliminarily identify 3311 NTM strains isolated from mycobacterium-positive culture samples from patients visiting tuberculosis designated hospitals in 8 suburban counties and districts in Hangzhou from January 2021 to December 2024.The species of NTM isolates were confirmed using mycobacterium molecular linear probe method and sequencing of PCR product of 16S rRNA,ITS and Hsp65 gene.Concentration proportional method was applied to detect the susceptibility of NTM isolates to isoniazid,rifampicin,streptomycin,ethambutol,ofloxacin,levofloxacin,moxifloxacin,and kanamycin.Results:PNB/TCH growth tests showed that 12.4％(409/3311)of the 3311 mycobacterium-positive culture samples were positive.Out of these 409 specimens preliminarily identified as NTM,22(5.4％)strains were Mycobacterium tuberculosis,19(4.6％)strains were non mycobacterial.The total number of NTM patients included in this study was 368.The mycobacterium molecular linear probe hybridization and PCR product sequencing confirmed that among these 368 NTM samples,22 types of nontuberculous mycobacteria were identified,60.6％(223/368)were identified as Mycobacterium intracellulare,followed by Mycobacterium abscessus(11.7％,43/368),Mycobacterium kansasii(6.5％,24/368),Mycobacterium avium(5.2％,19/368),Mycobacterium chelonei(4.6％,17/368)and Mycobacterium marseillense(2.7％,10/368).The resistance rates of 368 NTM isolates to isoniazid,rifampicin,streptomycin,ethambutol,ofloxacin,levofloxacin,moxifloxacin,and kanamycin were 99.2％(365/368),82.3％(303/368),93.5％(344/368),73.4％(270/368),87.2％(321/368),86.1％(317/368),85.1％(313/368),and 80.7％(297/368),respectively.Conclusion:Mycobacterium intracellulare is the predominant NTM species in Hangzhou suburban areas.Moreover,NTM isolates have high resistance against commonly used antituberculosis drugs.关键词
分枝杆菌属/非典型性细菌/抗药性/疾病易感性Key words
Mycobacterium/Atypical bacterial forms/Drug resistance/Disease susceptibility分类
医药卫生引用本文复制引用
吴亦斐,李清春,贾庆军,黄银燕,白雪欣,程庆林..杭州郊县区非结核分枝杆菌肺病病原菌鉴定及其耐药性分析[J].结核与肺部疾病杂志,2025,6(4):420-425,6.基金项目
浙江省基础公益研究计划项目(LGF21H190002) （LGF21H190002）
浙江省医药卫生科技计划项目(2021KY951 （）
2020KY238) （）
杭州市医药卫生科技计划项目(A20200280)The Public Welfare Technology Research Program in Zhejiang Province(LGF21H190002) （A20200280）
Zhejiang General Research Project on Medical Health and Science Technology Plan(2021KY951 （）
2020KY238) （）
The Hangzhou Medical and Health Science and Technology Project(A20200280) （A20200280）
