经济动物学报2025，Vol.29Issue(4)：299-304,6.DOI:10.13326/j.jea.2025.1834
山东省部分地区水貂阿留申病PCR检测及病原的分离鉴定
PCR Detection,Pathogen Isolation and Identification of Mink Aleutian Disease in Some Areas of Shandong Province
摘要
Abstract
In order to investigate the prevalence and biological characteristics of isolated strains of mink Aleutian disease virus(ADV)in Shandong Province,specimens were collected from five major mink breeding areas in Shandong Province,for PCR detection,isolation and cultivation of ADV,analysis of homology and genetic variation of isolated strains.The results showed that 1 917 bp specific band of ADV VP2 gene was obtained by PCR using ADV-specific primers.The positive rate of ADV in the mink farms of the five regions was 38.9%-67.1%.At the same time,a new strain of ADV,named ADV-SD1908,was isolated and identified,which exhibited significant cytopathic effects(CPE)on Crandell Reese Feline Kidney(CRFK)cells.TCID50 of the isolated strain was 10-3.64/mL.The homology between this strain and other strains is 94.4%-96.1%,and it has the closest genetic relationship with domestic HLJ strain and foreign Pullman strain.The research results will provide references for the epidemiology,genetic evolution,prevention and control of mink ADV in Shandong Province.关键词
水貂/阿留申病毒/分离鉴定/VP2基因/生物学特性Key words
mink/aleutian disease virus/isolation and identification/VP2 gene/biological characteristic分类
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张定秀,井长华,王若楠,朱毅松,李宏梅,王贵升,邱建华,郭慧君..山东省部分地区水貂阿留申病PCR检测及病原的分离鉴定[J].经济动物学报,2025,29(4):299-304,6.基金项目
山东省现代农业产业技术体系特种经济动物创新团队岗位科学家专项(SDAIT-21-10&SDAIT-21-5),国家林业和草原局中央本级专项(2130211) （SDAIT-21-10&SDAIT-21-5）
