江西农业学报2025，Vol.37Issue(8)：1-7,7.DOI:10.19386/j.cnki.jxnyxb.2025.08.001
苦瓜不同基因型、组织及发育阶段皂苷的变化特征研究
Study on Variation Characteristics of Saponins in Different Bitter Gourd Genotypes,Tissues and Developmental Stages
摘要
Abstract
The vanillin-glacial acetic acid method was used to determine and compare the total saponin content in different bitter gourd genotypes(MC1～MC11)and MC10 tissues(roots,stems,leaves,flowers,fruits and seeds).High-performance liquid chromatography(HPLC)was employed to analyze the types of saponin monomers in leaves,fruits and seeds and to determine the content of some monomers at different fruit development stages(ovary stage,young fruit stage,commercial fruit stage and full maturity stage),in order to provide a reference for the development and utilization of medicinal components in bitter gourd.The results showed that:(1)The content of saponins in fruits of 11 different bitter gourd inbred lines ranged from 0.18 g/100 g to 1.6 g/100 g,among which the genotype'MC6'had the highest total saponin content.In inbred genotype'MC10',the highest saponin content was detected in the flower tissue,with the level of 1.023 g/100 g,while the lowest content was in the stem tissue.(2)HPLC results showed that five saponin monomers(G,K,L,I and F1)were detected in the bitter gourd fruits,monomer G,K,L and I were detected in the leaves,and only monomers I and C were found in the seeds.(3)The saponin monomer G and K increased with the bitter gourd fruits ripening,and then decreased at late development stages.The highest content of monomer G and K were detected at young fruit stage and commercial fruit stage,respectively.关键词
苦瓜/皂苷/单体/基因型/高效液相色谱Key words
Bitter gourd/Saponin/Monomer/Genotype/High-performance liquid chromatography分类
农业科技引用本文复制引用
宁宇,陈龙正,戚仁洁,刘静,刘之洋,练华山,刘正鲁,吕珍璐,罗志丹,徐海..苦瓜不同基因型、组织及发育阶段皂苷的变化特征研究[J].江西农业学报,2025,37(8):1-7,7.基金项目
江苏省重点研发计划(现代农业)项目(BE2022339) （现代农业）
江苏省高效园艺作物遗传改良重点实验室项目(ZD2022003). （ZD2022003）
