江西农业学报2025，Vol.37Issue(8)：15-22,8.DOI:10.19386/j.cnki.jxnyxb.2025.08.003
大丽花原生质体PEG介导融合条件优化研究
Optimization of PEG-mediated Fusion Conditions of Dahlia pinnata Protoplasts
摘要
Abstract
The study systematically optimized the protoplast fusion system induced by the PEG-high Ca2+high pH method using the leaves and petals of Dahlia imperialis and'Café au Lait Royal'as test materials.The results showed that when the protoplast density was adjusted to 3.0×105 protoplasts/mL,the fusion rate of leaf and petal protoplasts could reach 15.57%when they were treated with 30%PEG-4000 and 20%PEG-6000,respectively,for 25 min,then added with a high Ca2+-high pH solution and left standing at room temperature for 10 min.PEG fusion by protoplasts from different sources showed that the fusion rate of dahlia protoplasts could reach 5.70%.This study shows for the first time that the protoplasts from different tissue sources of dahlia have specific responses to the molecular weight and concentration of PEG,and the established differential parameter system can provide technical support for somatic cell hybrid breeding of self-pollinated crops.关键词
大丽花/原生质体/PEG/体细胞杂交Key words
Dahlia pinnata/Protoplast/PEG/Somatic hybridization分类
农业科技引用本文复制引用
周静,杨冬,葛思怡,周锦民,田震,魏怡,田洋波,吴学尉..大丽花原生质体PEG介导融合条件优化研究[J].江西农业学报,2025,37(8):15-22,8.基金项目
云南省重大科技专项(农业)基金项目"大丽花与矮牵牛种质创新研究"(202102AE090052) （农业）
云南大学第四届专业学位研究生实践创新"大丽花原生质体培养及遗传转化体系构建"(ZC-24248890). （ZC-24248890）
