江西农业学报2025，Vol.37Issue(8)：48-56,9.DOI:10.19386/j.cnki.jxnyxb.2025.08.008
不同肥力梯度对红壤地春播青贮玉米土壤微生物群落组成的影响
Impact of Different Fertility Gradients on Composition of Soil Microbial Communities in Spring-sown Silage Maize Fields under Red Soil Conditions
摘要
Abstract
This study investigated the effects of different application methods on soil microbial diversity and functional microbial structure in silage maize by soil microbial sequencing technology through a field experiment with a two-factor orthogonal design:compound fertilizer(C1～C3:300,525,750 kg/hm2)and urea(N1～N3:150,300,450 kg/hm2),including a no-fertilizer control(CK).The results showed that in bacterial diversity,the Shannon index of the C2N3 treatment was significantly lower than that of CK.Simpson index decreased significantly in C2N2 and C1N3,while the Chao1 index of C1N3 was significantly higher than that of C3N1 and C3N3.The Chao1 and Shannon indices of C2N2 were significantly elevated compared to C3N2(CK),and C1N2 in fungi.In community structure differences,PCoA revealed significant divergence between fertilized and CK groups in bacteria.Distinct clustering was observed between C3N1 and C1N1 in fungi.Proteobacteria,Acidobacteriota,and Actinobacteriota were dominant taxa.Ascomycota and Basidiomycota were predominant.LEfSe analysis showed that Acidobacteriota was enriched in C3N3,Proteobacteria in C3N1,Cyanobacteria in C2N3,and Solirubrobacter in C1N1.In fungi,Penicillium was enriched in C2N2,and Aspergillus flavus in C3N2.Tax4Fun functional analysis revealed that carbohydrate metabolism,metabolism of other amino acids,metabolism of cofactors and vitamins,xenobiotics biodegradation and metabolism,signal transduction and environmental adaptation have higher abundance in fertilization treatment groups.FUNGuild functional analysis revealed that the samples had high abundance of saprophytic and pathotrophic-saprophytic-symbiotic fungal trophic groups.Overall,the C2N2 treatment(525 kg/hm2 compound fertilizer+300 kg/hm2 urea)was the best fertilization method for silage corn in the red soil area.关键词
土壤微生物/复合肥/尿素/红壤/青贮玉米Key words
Soil microorganisms/Compound fertilizer/Urea/Red soil/Silage maize分类
农业科技引用本文复制引用
彭旭艺,徐桂花,高朝煜,刘水华,顾瑶,黄栋,刘继明..不同肥力梯度对红壤地春播青贮玉米土壤微生物群落组成的影响[J].江西农业学报,2025,37(8):48-56,9.基金项目
江西省牛羊产业技术体系(JXARS-13). （JXARS-13）
