检验医学与临床2025，Vol.22Issue(14)：1904-1908,1914,6.DOI:10.3969/j.issn.1672-9455.2025.14.007
结肠癌中MLH-1、MSH-2、MSH-6、PMS-2蛋白表达情况及临床意义
Expression and clinical value of MLH-1,MSH-2,MSH-6,PMS-2 protein in colon cancer
摘要
Abstract
Objective To investigate the relationship between the expression of mismatch repair proteins Mut L homologous protein(MLH)-1,Mut S homolog(MSH)-2,MSH-6,post meiotic separation protein 2(PMS-2)protein in colon cancer and the clinical characteristics,prognosis of patients.Methods A total of 150 colon cancer patients who visited the Department of Gastrointestinal surgery in the General Hospital of Anga-ng Group from June 2021 to June 2022 were collected as the research subjects.The expression of MLH-1,MSH-2,MSH-6 and PMS-2 protein in colon cancer tissues and adjacent tissues was detected by immunohisto-chemistry.If at least one of the four mismatch repair proteins was detected to be negative in expression,it was classified as a mismatch repair gene defect(dMMR)group,if all mismatch repair proteins were judged posi-tive,it was classified as a complete mismatch repair gene(pMMR)group.The clinical characteristics and sur-vival rates of the two groups were compared.Results MLH-1,MSH-2,MSH-6 and PMS-2 protein were all located in the nucleus of the cell.The protein loss rate of MLH-1,MSH-2,MSH-6 and PMS-2 in colon cancer tissues were higher than those in adjacent tissues,the differences were statistically significant(P<0.05).A-mong the 150 patients,pMMR accounted for 80.67%,and dMMR group accounted for 19.33%.The protein expression single loss rates of MLH-1,MSH-2,MSH-6 and PMS-2 among 150 colon cancer patients were 4.00%,2.67%,2.00%and 6.00%respectively.Among them,the simultaneous protein expression loss rates of MLH-1+PMS-2 and MSH-2+MSH-6 were 3.33%and 1.33%respectively,and no cases missing all of four proteins was found.The proportion of mucinous adenocarcinoma,incidence in the right colon and clinical stage Ⅰ+Ⅱ in the dMMR group were significantly higher than those in the pMMR group,the differences were statistically significant(P<0.05).The 2-year follow-up survival rate of the dMMR group was 48.27%,which was significantly higher than the 28.92%of the pMMR group,the difference was statistically signifi-cant(P<0.05).Conclusion The expression of four mismatch repair proteins including MLH-1,MSH-2,MSH-6 and PMS-2 in colon cancer is related to clinical characteristics such as pathological tissue classifica-tion,staging and tumor location.Patients with dMMR colon cancer have better prognosis than those with pM-MR colon cancer.MLH-1,MSH-2,MSH-6 and PMS-2 protein can be important indicators for prognosis of co-lon cancer.关键词
结肠癌/Mut L同源蛋白/减数分裂后分离蛋白2/错配修复/临床特征/预后Key words
colon cancer/Mut L homologous proteins/postmeiotic protein isolate 2/mismatch re-pair/clinical features/prognosis分类
医药卫生引用本文复制引用
刘嘉文,李博,吴国刚,田学昌,姚学清..结肠癌中MLH-1、MSH-2、MSH-6、PMS-2蛋白表达情况及临床意义[J].检验医学与临床,2025,22(14):1904-1908,1914,6.基金项目
国家自然科学基金项目(82260501). （82260501）
