检验医学与临床2025，Vol.22Issue(14)：1915-1919,1925,6.DOI:10.3969/j.issn.1672-9455.2025.14.009
区域淋巴结对结肠癌生长及休眠的影响
Effect of regional lymph nodes on tumor growth and dormancy of colonic cancer
摘要
Abstract
Objective To explore the effect of regional lymph nodes on tumor growth and dormancy of co-lonic cancer in mice.Methods Twelve female BALB/c mice were selected as the research subjects and ran-domly divided into a regional lymph node resection group(Group A)and a regional lymph node preservation group(Group B)using the coin toss method,with six mice in each group.Group A underwent regional lymph node resection+CT26.WT injection,while Group B underwent virtual surgery+CT26.WT injection.Dynami-cally observe the growth of transplanted tumors of the two groups,and dissect the transplanted tumors after ectopic growth.Compare the volume and weight of the transplanted tumors between the two groups of mice.Ki-67 immunohistochemistry was used to detect nuclear staining rate,Western blotting was used to detect the level of sleep marker P21 protein,and flow cytometry was used to detect the cell cycle.Results On the 28th day of the experiment,both groups of mice had developed ectopic transplanted tumors.The volume and weight of the transplanted tumors in Group A were(1 217.27±77.04)mm3 and(0.75±0.03)g respectively,while those in Group B were(463.85±12.85)mm3 and(0.28±0.03)g respectively.The volume and weight of the transplanted tumors in Group A were significantly larger and more heavy than those in Group B,the differ-ences were statistically significant(P<0.05).Neither group of mice showed lymph node or organ metastasis.The P21/β-actin ratio in Group A was 0.76±0.09,the P21/β-actin ratio in Group B was 0.95±0.15,the level of P21/β-actin in group B was significantly higher than that in group A,the difference was statistically signifi-cant(t=2.419,P=0.036).The proportion of G0/G1 phase cells in Group A and Group B were(51.84±1.75)%and(58.65±2.56)%respectively,the proportion of G0/G1 phase cells in Group B was significantly higher than that in Group A,the difference was statistically significant(t=5.49,P<0.001).Conclusion Re-gional lymph nodes may promote tumor dormancy by exerting anti-tumor immunity,thereby inhibiting tumor growth,the regulation of dormancy of colonic cancer by regional lymph nodes may provide new ideas for tumor treatment.关键词
区域淋巴结/结肠癌/肿瘤休眠/P21/肿瘤免疫Key words
regional lymph nodes/colonic cancer/tumor dormancy/P21/tumor immanity分类
医药卫生引用本文复制引用
刘国吾,黄冰,黄桂林,刘晓婵,侯吉学..区域淋巴结对结肠癌生长及休眠的影响[J].检验医学与临床,2025,22(14):1915-1919,1925,6.基金项目
新疆生产建设兵团科技计划项目(2022ZD025) （2022ZD025）
天山英才项目(2023TSYCTD0020). （2023TSYCTD0020）
