检验医学与临床2025，Vol.22Issue(15)：2038-2043,6.DOI:10.3969/j.issn.1672-9455.2025.15.005
不同Pfirrmann分级的腰椎间盘突出症患者血清CXCL10、Sirtuin1水平及临床意义
Serum CXCL10 and Sirtuin1 levels and their clinical significance in patients with lumbar disc herniation of different Pfirrmann classification
摘要
Abstract
Objective To analyze the levels of serum CXC chemokine ligand 10(CXCL10)and silent infor-mation regulator 1(Sirtuin1)and their clinical significance in patients with lumbar disc herniation(LDH)of different Pfirrmann classification.Methods A total of 150 LDH patients admitted to the hospital from April 2023 to April 2024 were selected as the study group,and 150 healthy volunteers who underwent physical ex-amination in the hospital during the same period were selected as the control group.The patients were divided into Ⅰ-Ⅴ classes according to Pfirrmann classification.The serum levels of CXCL10,Sirtuin1,interleukin-6(IL-6)and matrix metalloproteinase-3(MMP-3)were detected by enzyme-linked immunosorbent assay.Pear-son or Spearman correlation analysis was used to analyze the correlation between serum CXCL10,Sirtuin1 lev-els and IL-6,MMP-3 levels,visual analogue scale(VAS)score,Oswestry disability index(ODI)score and Pfirrmann classification in patients with LDH and the correlation between serum CXCL10 and Sirtuin1 levels in patients with LDH.Multivariate Logistic regression was used to analyze the influencing factors of LDH.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum CX-CL10 and Sirtuin1 for LDH.Results The serum levels of CXCL10,IL-6 and MMP-3 in the study group were higher than those in the control group,and the serum level of Sirtuin1 was lower than that in the control group,and the differences were statistically significant(P<0.05).The comparison of serum CXCL10,IL-6,and MMP-3 levels in patients with different Pfirrmann classification of LDH showed that class Ⅰ-Ⅱ<classⅢ<class Ⅳ<class Ⅴ,and any pairwise comparison showed statistically significant differences(P<0.05).The comparison of serum Sirtuin1 levels in patients with different Pfirrmann classification of LDH showed that class Ⅰ-Ⅱ>class Ⅲ>class Ⅳ>class Ⅴ,and any pairwise comparison was statistically significant(P<0.05).The VAS and ODI scores of patients with Pfirrmann class V were higher than those of patients with Pfirrmann class Ⅰ-Ⅱ,Ⅲ and Ⅳ,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that serum CXCL10 level in LDH patients was negatively correlated with Sirtuin1 level,and positively correlated with IL-6 and MMP-3 levels,VAS and ODI scores(P<0.05).Serum Sirtuin1 level was negatively correlated with IL-6 and MMP-3 levels,VAS and ODI scores(P<0.05).Spearman cor-relation analysis showed that Pfirrmann classification of LDH patients was positively correlated with serum CXCL10 level(P<0.05),and negatively correlated with serum Sirtuin1 level(P<0.05).Multivariate Logis-tic regression analysis showed that the increased serum levels of CXCL10,IL-6,and MMP-3 were risk factors for the occurrence of LDH(P<0.05),and the increased serum level of Sirtuin1 was a protective factor for the occurrence of LDH(P<0.05).ROC curve analysis showed that the area under the curve(AUC)of the combination of the two indicators in the diagnosis of LDH was 0.945,which was greater than the AUC of serum CXCL10 and Sir-tuin1 alone(Z=2.715,2.722;both P<0.05).Conclusion The Pfirrmann classification of LDH patients is related to the serum levels of CXCL10 and Sirtuin1,which can be used as biomarkers for the diagnosis of LDH.关键词
腰椎间盘突出症/Pfirrmann分级/CXC趋化因子配体10/沉默信息调节因子1/相关性Key words
lumbar disc herniation/Pfirrmann classification/CXC chemokine ligand 10/silent infor-mation regulator 1/correlation分类
医药卫生引用本文复制引用
卫江文,孙光伟,郭小亮,宋延荣..不同Pfirrmann分级的腰椎间盘突出症患者血清CXCL10、Sirtuin1水平及临床意义[J].检验医学与临床,2025,22(15):2038-2043,6.基金项目
2021年山西省"四个一批"科技兴医创新计划项目(2020XM51). （2020XM51）
