检验医学与临床2025，Vol.22Issue(15)：2054-2058,2065,6.DOI:10.3969/j.issn.1672-9455.2025.15.008
CAMK1与p62在直肠癌中的表达及其与临床病理特征的关系研究
Study on the expression of CAMK1 and p62 in rectal cancer and their relationship with clinicopathological characteristics
摘要
Abstract
Objective To analyze the expression of calcium/calcineprotein-dependent kinase 1(CAMK1)and autophagy-related protein(p62)in rectal cancer and their relationship with clinicopathological characteris-tics.Methods A retrospective selection was made of 80 patients with rectal cancer admitted to Weinan Cen-tral Hospital from October 2022 to July 2023 as the research subjects.Postoperative cancer tissues and corre-sponding adjacent tissue pathological specimens were taken.The positive rates and the level of messenger RNA(mRNA)of CAMK1 and p62 in tissue specimens were detected by immunohistochemical staining and real-time fluorescence quantitative polymerase chain reaction.The expression of CAMK1 and p62 in rectal cancer patients with different clinicopathological characteristics was compared,and the correlation between the level of CAMK1 mRNA and the level of P62 mRNA in rectal cancer tissues was analyzed by Pearson correla-tion.Results The immunohistochemical staining results showed that CAMK1 was mainly expressed in the cytoplasm and nucleus,and p62 was mainly expressed in the nucleus.The positive rate of CAMK1 and the lev-el of CAMK1 mRNA in rectal cancer tissues were significantly lower than those in adjacent tissues,while the positive rate of p62 and the level of p62 mRNA in rectal cancer tissues were significantly higher than those in adjacent tissues,and the differences were statistically significant(P<0.05).In rectal cancer patients,the posi-tive rate of CAMK1 in cancer tissues of patients with TNM stage Ⅲ-Ⅳ was lower than that of patients with stage Ⅰ-Ⅱ,and the positive rate of CAMK1 in cancer tissues of patients with lymph node metastasis was lower than that of patients without lymph node metastasis,and the differences were statistically signifi-cant(P<0.05).Among rectal cancer patients,the positive rate of p62 in those with intestinal wall invasion depth of T3-T4 was higher than that of patients with T1-T2,the positive rate of p62 in those with TNM stage Ⅲ-Ⅳ was higher than that of patients with stage Ⅰ-Ⅱ,and the positive rate of p62 in those with lymph node metastasis was higher than that of patients without lymph node metastasis,and the differences were all statistically significant(P<0.05).The results of Pearson correlation analysis showed that the level of CAMK1 mRNA in rectal cancer tissues was negatively correlated with the level of p62 mRNA(P<0.05).Conclusion CAMK1 is lowly expressed and p62 is highly expressed in rectal cancer tissues.The expressions of the two are negatively correlated and both are closely related to clinicopathological characteristics.They can be used as new biomarkers for the diagnosis of rectal cancer.关键词
直肠癌/钙/钙调蛋白依赖性激酶/自噬相关蛋白/临床病理特征/相关性Key words
rectal cancer/calcium/calmodulin-dependent kinase/autophagy-related protein/clinico-pathological characteristics/correlation分类
医药卫生引用本文复制引用
刘静,杨小平,王佩,刘囡..CAMK1与p62在直肠癌中的表达及其与临床病理特征的关系研究[J].检验医学与临床,2025,22(15):2054-2058,2065,6.基金项目
陕西省自然科学基础研究计划项目(2022JM-582). （2022JM-582）
