检验医学与临床2025，Vol.22Issue(15)：2066-2070,5.DOI:10.3969/j.issn.1672-9455.2025.15.010
miR-200/Nrf2在胎膜早破中的调控作用
Regulation of miR-200/Nrf2 in premature rupture of membranes
摘要
Abstract
Objective To analyze the regulatory role of miR-200/nuclear factor erythroid 2-related factor 2(Nrf2)in premature rupture of membranes(PROM)based on bioinformatics.Methods A total of 200 pa-tients with PROM before full-term delivery in the Fourth Hospital of Shijiazhuang from September 2021 to October 2023 were selected as the PROM group,and 200 healthy pregnant women who underwent physical examination in the Fourth Hospital of Shijiazhuang during the same period were selected as the control group.Hematoxylin-eosin(HE)staining was used to detect the histological changes of fetal membranes in the two groups.The database was used to screen the microRNA(miRNA)targeting Kelch-like ECH-associated pro-tein 1(Keap1)gene and Nrf2 co-expressed genes.The expression levels of miR-200,Keap1 and Nrf2 in fetal membranes of the two groups were detected by real-time fluorescent quantitative polymerase chain reaction(qPCR),and the expression of Nrf2 was identified by immunohistochemistry.Results The fetal membranes of the control group showed normal structure,while the fetal membranes of the PROM group showed obvious inflammation,necrosis and fibrosis.The results of database screening showed that miR-141-3p and miR-200a-3p could target Keap1.The expression levels of miR-200 and Keap1 in fetal membranes of the PROM group were higher than those of the control group,and the expression level of Nrf2 was lower than that of the con-trol group,and the differences were statistically significant(P<0.05).The expression levels of Nrf2 in the amniotic epithelial layer connective tissue layer,villous trophoblast layer,and decidual layer in the PROM group were lower than those in the control group,and the differences were statistically significant(P<0.05).MEM database results showed that Nrf2 and its co-expressed genes were mainly involved in the negative regu-latory biological processes of protein kinase R-like endoplasmic reticulum kinase-mediated unfolded protein re-sponse,serine dephosphorylation,and endoplasmic reticulum stress-induced eukaryotic initiation factor 2α sub-unit phosphorylation.Conclusion miR-200/Nrf2 may play an important regulatory role in the development of PROM before term labor.miR-200 may be involved in activating Keap1,inhibiting Nrf2 expression,weakening antioxidant and anti-inflammatory ability,eventually leading to inflammation,necrosis and fibrosis of fetal membranes,and increasing the risk of PROM before term labor.关键词
胎膜早破/微小RNA-200/核因子E2相关因子2/胎膜组织/生物调控Key words
premature rupture of membranes/microRNA-200/nuclear factor E2-related factor 2/fe-tal membrane tissue/biological regulation分类
医药卫生引用本文复制引用
韩乐,刘鹏,张璇,高峰,张雪玲..miR-200/Nrf2在胎膜早破中的调控作用[J].检验医学与临床,2025,22(15):2066-2070,5.基金项目
河北省石家庄市科技计划项目(221460783). （221460783）
