皮肤性病诊疗学杂志2025，Vol.32Issue(7)：512-517,6.DOI:10.3969/j.issn.1674-8468.2025.07.007
趋化因子CCL5在斑秃患者外周血中的表达水平研究
Study on expression levels of chemokine CCL5 in peripheral blood of patients with alopecia areata
摘要
Abstract
Objective To explore the role of C-C motif chemokine ligand 5(CCL5)in the pathogenesis of alopecia areata,by detecting the expression levels of C-C motif chemokine ligand 5(CCL5)in peripheral blood mononuclear cells(PBMCs)and serum of patients with alopecia areata at different disease severities,durations,and stages.Methods A total of 58 patients were selected as the study group,and 30 healthy people from the physical examination center served as controls.Expression levels of CCL5 mRNA in PBMCs were detected by RT-PCR,and CCL5 levels in serum were measured by ELISA.Expression levels of CCL5 mRNA in PBMCs and CCL5 levels in serum were compared among alopecia areata patients with different disease severities,dura-tions,and stages,and their correlation with SALT scores was assessed.Results Expression levels of CCL5 mRNA in PBMCs were significantly higher in alopecia areata patients than in controls(Z=-2.95,P＜0.05).Patients with severe alopecia areata showed higher expression levels of CCL5 mRNA than those with mild disease and controls.Group with disease duration of either＞6-month or ≤6-month had higher levels of CCL5 mRNA than the control group,and patients in the active phase had higher expression levels than those in the stable phase and controls(all P＜0.05).However,no significant differences were found between the mild group and controls,be-tween the＞6-month and ≤6-month groups,or between the stable phase group and controls(all P＞0.05).Comparison of serum CCL5 levels in peripheral blood revealed that the alopecia areata group had significantly higher levels than controls(Z=-3.72,P＜0.001).Patients with severe alopecia areata had higher levels than controls,and both active and stable phase patients showed elevated levels compared to controls.Patients with disease duration ≤6-months also had higher levels than controls(all P＜0.05).However,no significant differences were observed between the mild and severe groups,between active and stable phase groups,between＞6-month and ≤6-month duration groups,or between these groups and controls(all P＞0.05).Expression levels of CCL5 mRNA in PBMCs of alopecia areata patients were positively correlated with SALT scores(r=0.46,P＜0.001),and serum CCL5 levels were also positively correlated with SALT scores(r=0.32,P=0.021).Conclusions CCL5 is correlated with the severity,duration,and clinical stage of alopecia areata,and may serve as a potential biomarker for evaluating treatment efficacy.关键词
斑秃/C-C类趋化因子配体5/外周血单个核细胞Key words
alopecia areata/CCL5/PBMCs引用本文复制引用
谭喻丹,何芊芊,肖雨菲,徐子涵,晏文,杨莹..趋化因子CCL5在斑秃患者外周血中的表达水平研究[J].皮肤性病诊疗学杂志,2025,32(7):512-517,6.基金项目
遵义市科学技术基金项目[遵市科合HZ字(2024)186号] （2024）
贵州省基础研究计划自然科学项目[黔科合基础-ZK(2022)一般668] （2022）
