现代畜牧兽医Issue(7)：1-5,5.DOI:10.20154/j.cnki.issn1672-9692.2025.07.001
泰拉霉素平衡溶解度和表观油水分配系数的测定
Determination of equilibrium solubility and apparent oil-water partition coefficient of tulathromycin
摘要
Abstract
This study aimed to determine the equilibrium solubility of tulathromycin in phosphate buffers at different pH values and its apparent oil-water partition coefficient(Pow)in n-octanol/water and buffer systems,providing references for developing new tulathromycin formulations.A high-performance liquid chromatography(HPLC)method was established and validated for tulathromycin quantification through precision,stability,repeatability,and recovery tests.The equilibrium solubility of tulathromycin was measured in phosphate buffers(pH 1.0～8.0)and ultrapure water,while the apparent Pow was determined using the shake-flask method in n-octanol/buffer systems across varying pH levels.Results demonstrated that tulathromycin was very slightly soluble in pH 1.0 phosphate buffer,practically insoluble at pH 4.5,5.8,and 6.8 buffers,and insoluble at pH 7.4,8.0 buffers and ultrapure water.The logarithm of Pow(lgPow)ranged between 0 and 1.The established HPLC method exhibited excellent specificity for tulathromycin quantification.Tulathromycin demonstrated poor water solubility with moderate lipophilicity,and both its equilibrium solubility and Pow were pH-dependent,suggesting favorable biomembrane permeability.关键词
泰拉霉素/高效液相色谱法/平衡溶解度/表观油水配系数Key words
Tulathromycin/High-performance liquid chromatography(HPLC)/Equilibrium solubility/Apparent oil-water partition coefficient分类
农业科技引用本文复制引用
钟雅文,李子杨,魏知非,陈佳琪,吴春璇,相亦飞,何家康..泰拉霉素平衡溶解度和表观油水分配系数的测定[J].现代畜牧兽医,2025,(7):1-5,5.基金项目
广西重点研发计划项目重点专项(桂科AB23026082) （桂科AB23026082）
大学生创新创业训练计划项目(202210593097) （202210593097）
