现代畜牧兽医Issue(7)：11-16,6.DOI:10.20154/j.cnki.issn1672-9692.2025.07.003
猪源雷氏普罗威登斯菌辽宁株的分离鉴定及其耐药性分析
Isolation,identification,and drug resistance analysis of Providencia rettgeri Liaoning strain from swine
摘要
Abstract
This study aimed to investigate the pathogenicity and drug resistance of Providencia rettgeri isolated from pigs in Liaoning Province.Tissue samples(liver,intestine)were aseptically collected from diseased piglets on a farm in Yingkou City,Liaoning.Pathogen identification was performed using bacterial isolation/culture and molecular biology techniques,followed by mouse pathogenicity assays and antimicrobial susceptibility testing.Results showed that a strain of Providencia rettgeri was successfully isolated.Colonies appeared gray-white,moist,convex,and medium-sized on 10%sheep blood agar without hemolysis.Gram staining revealed Gram-negative short bacilli with rounded ends.Inoculation caused 100%morbidity and 50%mortality in mice,inducing pathological damage in multiple organs(liver,lungs,intestine),confirming strong pathogenicity.The isolate was sensitive to five antimicrobials:Ceftriaxone,amikacin,ciprofloxacin,norfloxacin,and levofloxacin,intermediate to cefoperazone and gentamicin,and resistant to ten others(including ampicillin,cefazolin,and ceftazidime).The study reveals that the Providencia rettgeri isolated from pigs in Liaoning Province has developed resistance to most antimicrobial agents.However,it remains highly susceptible to five antimicrobials:ceftriaxone(no approved veterinary formulation),amikacin,norfloxacin,and levofloxacin(internationally banned for veterinary use),leaving only ciprofloxacin as a clinically applicable veterinary drug for prevention and treatment.These findings provide valuable references for developing control strategies against Providencia rettgeri infections in swine.关键词
猪/雷氏普罗威登斯菌/分离鉴定/致病性/耐药性/辽宁株Key words
Swine/Providencia rettgeri/Isolation and identification/Pathogenicity/Drug resistance/Liaoning strain分类
农业科技引用本文复制引用
由莹,刘丽颖,周大勇,孙弋雅,任振堃,王辉暖,高慎阳,周铁忠..猪源雷氏普罗威登斯菌辽宁株的分离鉴定及其耐药性分析[J].现代畜牧兽医,2025,(7):11-16,6.基金项目
辽宁省科学技术计划农业重大专项(2023JH11020006) （2023JH11020006）
