现代畜牧兽医Issue(7)：17-21,5.DOI:10.20154/j.cnki.issn1672-9692.2025.07.004
乌鲁木齐市某海鲜市场海产品中副溶血性弧菌的分离与检测
Isolation and identification of Vibrio parahaemolyticus in seafood from a market in Urumqi
摘要
Abstract
This study assessed Vibrio parahaemolyticus contamination in seafood from a Urumqi market.Samples included 18 grouper tissues(heart,gills,liver,muscle,intestine,scales),20 swimming crab tissues(heart,stomach,muscle,gills),and 60 Pacific white shrimp(Litopenaeus vannamei)tissues(brain,muscle,intestinal tract).Bacterial isolation,biochemical identification,and PCR amplification targeting partial BL1S 16S rRNA gene sequences were conducted.Results indicated no Vibrio parahaemolyticus in crabs or groupers.Shrimp samples showed 30%positivity(18/60),with 23 colonies exhibiting circular,blue-green,translucent,turbid morphology and 100%biochemical confirmation(23/23).All 18 non-replicate isolates were confirmed as Vibrio parahaemolyticus,showing tissue-specific distribution:Muscle 30%(6/20),intestinal tract 15%(3/20),brain 45%(9/20).The study confirms shrimp contamination in this market,while crabs and groupers remained uncontaminated.关键词
副溶血性弧菌/南美白对虾/细菌分离鉴定/海产品/乌鲁木齐市Key words
Vibrio parahaemolyticus/Pacific white shrimp(Litopenaeus vannamei)/Bacterial isolation and identification/Seafood/Urumqi分类
农业科技引用本文复制引用
王宏丹,热沙来提·吐孙江,阿卜杜克尤木·如则麦麦提,阿卜力孜·阿卜都热合曼,如鲜古丽·奥布力,凯迪日耶·艾孜热提玉麦尔,买买提·艾孜子..乌鲁木齐市某海鲜市场海产品中副溶血性弧菌的分离与检测[J].现代畜牧兽医,2025,(7):17-21,5.基金项目
新疆农业大学2024年度大学生创新训练计划项目(dxscx2024449) （dxscx2024449）
