青岛大学学报（医学版）2025，Vol.61Issue(3)：327-330,4.DOI:10.11712/jms.2096-5532.2025.61.081
ACSL4对MPP+处理后N2a细胞线粒体数量的影响
Effect of acyl-CoA synthetase long-chain family member 4 on mitochondrial number in N2a cells after 1-methyl-4-phenylpyridinium treatment
摘要
Abstract
Objective To investigate the effect of acyl-CoA synthetase long-chain family member 4(ACSL4)on mitochon-drial number in N2a cells after 1-methyl-4-phenylpyridinium(MPP+)treatment.Methods Western blotting was used to mea-sure the protein expression level of ACSL4 in N2a cells after MPP+treatment.N2a cells were transfected with Flag-Vector or Flag-ACSL4 plasmids,and Western blotting was used to observe the effect of ACSL4 overexpression on the expression levels of voltage-dependent anion channel 1(VDAC1)and mitochondrial import receptor subunit TOM20 homolog(TOMM20)in N2a cells after MPP+treatment.Results Compared with the control group,the MPP+treatment group had a significant increase in the protein expression level of ACSL4 in N2a cells(t=3.965,P＜0.05).Compared with the control group,the MPP+treatment group had significant increases in the expression levels of VDAC1 and TOMM20(F=28.59,15.15,P＜0.01),while compared with the MPP+treatment group,ACSL4 overexpression had no further impact on the expression levels of VDAC1 and TOMM20 in N2a cells(P＞0.05).Conclusion ACSL4 overexpression has no impact on mitochondrial number in N2a cells after MPP+treat-ment,suggesting that the role of ACSL4 in the pathogenesis of Parkinson's disease does not involve its impact on mitochondrial number.关键词
辅酶A连接酶类/1-甲基-4-苯基吡啶/线粒体/电压依赖性阴离子通道1/帕金森病Key words
coenzyme A ligases/1-methyl-4-phenylpyridinium/mitochondria/voltage-dependent anion channel 1/Parkin-son disease分类
医药卫生引用本文复制引用
刘思齐,刘孟茹,姜宏..ACSL4对MPP+处理后N2a细胞线粒体数量的影响[J].青岛大学学报（医学版）,2025,61(3):327-330,4.基金项目
国家自然科学基金面上项目(32371013) （32371013）
中国博士后科学基金面上项目(2022M721754) （2022M721754）
