青岛大学学报（医学版）2025，Vol.61Issue(3)：345-349,5.DOI:10.11712/jms.2096-5532.2025.61.097
人参皂苷Rg1对TGF-β1诱导的星形胶质细胞反应性特性及细胞表型的影响
Effect of ginsenoside Rg1 on TGF-β1-induced reactivity and phenotype of astrocytes
摘要
Abstract
Objective To investigate the effect of ginsenoside Rg1 on the reactive characteristics and phenotypic transforma-tion of astrocytes(As)induced by transforming growth factor-beta 1(TGF-β1).Methods As were isolated from the cerebral cortex of neonatal Sprague-Dawley rats and were induced into reactive astrocytes(RAs)by TGF-β1,and then RAs were treated with ginsenoside Rg1 for 12 or 24 hours.The experiment was divided into control group,TGF-β1 group,and TGF-β1+Rg1 group.Quantitative real-time PCR was used to measure the mRNA expression levels of reactivity markers(Gfap,S100),inflammatory factors(IL-1β,TNF-α,iNOS),A1-type As markers(Gbp2,H2-T23),and A2-type As markers(S100A10,Ptx3),and Wes-tern blotting was used to measure the changes in the protein expression levels of GFAP and iNOS.Results Ginsenoside Rg1 sig-nificantly reduced the mRNA expression levels ofGfap,S100,iNOS,TNF-α,and IL-1β(F=11.09-136.70,q=6.43-16.01,P＜0.05)and the protein expression levels of GFAP and iNOS in RAs(F=8.67,7.66,q=6.80,5.36,P＜0.05).Compared with the control group,the TGF-β1+Rg1 group had significant reductions in the A1-type markers Gbp2 and H2-T23(F=61.94,18.46,q=13.95,5.58,P＜0.05)and significant increases in the A2-type markers S100A10 and Ptx3(F=25.46,31.11,q=7.70,10.65,P＜0.01).Compared with the TGF-β1 group,the TGF-β1+Rg1 group had significant reductions in the A1-type markers Gbp2 and H2-T23(F=61.94,18.46,q=13.29,8.45,P＜0.01),with no significant changes in A2-type markers(S100A10,Ptx3).Conclusion Ginsenoside Rg1 can inhibit TGF-β1-induced reactive characteristics of As and promotes their phenotypic transformation from A1 phenotype to A2 phenotype.关键词
人参皂苷Rg1/星形胶质细胞/转化生长因子β1/表型Key words
ginsenoside Rg1/astrocytes/transforming growth factor β1/phenotype分类
医药卫生引用本文复制引用
胡立荣,陈文芳,郑洋洋..人参皂苷Rg1对TGF-β1诱导的星形胶质细胞反应性特性及细胞表型的影响[J].青岛大学学报（医学版）,2025,61(3):345-349,5.基金项目
国家自然科学基金资助项目(31871144) （31871144）
山东省自然科学基金资助项目(ZR2022QH223) （ZR2022QH223）
