青岛大学学报（医学版）2025，Vol.61Issue(3)：359-362,4.DOI:10.11712/jms.2096-5532.2025.61.078
6-羟基多巴胺对BV2小胶质细胞铁蛋白表达的影响
Effect of 6-hydroxydopamine on the expression of ferritin in BV2 microglial cells
摘要
Abstract
Objective To investigate the effect of 6-hydroxydopamine(6-OHDA)on the expression of ferritin in BV2 mi-croglial cells.Methods After BV2 microglial cells were treated with different concentrations of 6-OHDA(10,25,50 μmol/L)for 24 hours,the amount of lactate dehydrogenase(LDH)released was measured to reflect cell viability,and Western blot was used to measure the changes in the protein expression levels of ferritin heavy chain 1(FTH1)and ferritin light chain(FTL)in cells.Results Compared with the control group without 6-OHDA treatment,the BV2 microglial cells treated with 10 μmol/L 6-OHDA for 24 hours had no significant change in the amount of LDH released,and the groups treated with 25 and 50 μmol/L 6-OHDA and the maximum enzyme activity control group had a significant increase in the amount of LDH released(F=92.750,q=6.799-24.780,P＜0.001).Compared with the control group,the BV2 microglial cells treated with 10 μmol/L 6-OHDA for 24 hours had significant increases in the protein expression levels of FTH1 and FTL(t=3.046,3.131,P＜0.05).Conclusion This study shows that 6-OHDA can increase the expression of ferritin in BV2 microglial cells.关键词
羟多巴胺/铁蛋白质类/小神经胶质细胞/帕金森病Key words
oxidopamine/ferritins/microglia/Parkinson disease分类
医药卫生引用本文复制引用
荆翠婷,李晓倩,曲艳,谢俊霞,王俊..6-羟基多巴胺对BV2小胶质细胞铁蛋白表达的影响[J].青岛大学学报（医学版）,2025,61(3):359-362,4.基金项目
山东省自然科学基金资助项目(ZR2021MC116) （ZR2021MC116）
