热带农业科学2025，Vol.45Issue(6)：56-64,9.DOI:10.12008/j.issn.1009-2196.2025.06.009
大杯蕈遗传多样性分析和DNA指纹图谱构建
Genetic Diversity Analysis and DNA Fingerprinting of Panus giganteus
摘要
Abstract
To explore the genetic diversity of Panus giganteus germplasm resources and accelerate the breeding process of high-quality mushroom varieties,10 strains of P.giganteus were used as experimental materials to measure their agronomic traits.Antagonistic tests and molecular marker analyses using ISSR,RAPD,and SRAP were conducted to assess genetic di-versity.DNA fingerprinting was performed based on the amplification results of the three molecular markers.Cluster analysis of agronomic traits revealed that the 10 strains were divided into four groups at a Euclidean distance of 2.5.The 8 ISSR prim-ers,10 RAPD primers,and 12 pairs of SRAP primers amplified 111,121,and 166 clear bands,respectively,with polymorphism ratios of ISSR(81.98％)＞SRAP(77.71％)＞RAPD(66.12％).Comprehensive clustering based on three molecular markers re-vealed that the 10 strains were divided into four groups at a genetic similarity coefficient of 0.76,with strains of similar ge-netic backgrounds clustering together.The tested strains exhibited rich genetic diversity,and the clustering results based on ISSR,RAPD,and SRAP markers were consistent with those of agronomic traits and antagonistic tests.ISSR molecular mark-ers are more consistent with the genetic background and can be used for DNA fingerprinting.These findings provide evidence for the identification and genetic relationship analysis of P.giganteus.关键词
大杯蕈/分子标记/遗传多样性/DNA指纹图谱Key words
Panus giganteus/molecular marker/genetic diversity/DNA fingerprint分类
农业科技引用本文复制引用
吴碧君,邱春锦,卢翠香,郑永德,曾丽兰,刘小英..大杯蕈遗传多样性分析和DNA指纹图谱构建[J].热带农业科学,2025,45(6):56-64,9.基金项目
福建省科技计划项目(No.2018N0040). （No.2018N0040）
