热带农业科学2025，Vol.45Issue(6)：65-71,7.DOI:10.12008/j.issn.1009-2196.2025.06.010
兜唇石斛离体快繁体系的建立
Establishment of an In vitro Rapid Propagation System for Dendrobium aphyllum
摘要
Abstract
Using the intact,crack-free capsules of wild Dendrobium aphyllum from Lincang,Yunnan Province,as the mate-rial,this study investigated aseptic germination,protocorm induction,tufted bud induction and proliferation,rooting and ac-climatization,and transplanting.The growth of Dendrobium aphyllum was compared on aseptic germination medium,aseptic seedling proliferation medium,and tufted bud rooting medium,supplemented with different concentrations of organic addi-tives,inorganic salts,and plant growth regulators.The optimal medium formula for each culture stage was selected to establish an in vitro rapid propagation system for D.aphyllum.The results revealed that the optimal medium for aseptic seed germina-tion was MS+potato juice(60 g·L-1)+sucrose(30 g·L-1)+agar(6.0 g·L-1).The optimal medium for tufted bud proliferation was MS+potato juice(60 g·L-1)+6-BA(0.5 mg·L-1)+NAA(0.2 mg·L-1)+AC(0.2 g·L-1),with a proliferation coefficient of 4.6.The optimal medium for robust seedling and rooting was 1/2 MS+banana juice(60 g·L-1)+IBA(0.5 mg·L-1)+6-BA(0.2 mg·L-1)+NAA(1.0 mg·L-1)+AC(0.2 g·L-1).Seedlings exhibited robust growth and well-developed root systems,with an average plant height of 3.28 cm,an average number of roots per plant of 15.6,and an average root length of 2.15 cm.The survival rate reached 100％ after 30 days of acclimatization and transplanting.关键词
兜唇石斛/离体培养/无菌萌发/增殖/生根Key words
Dendrobium aphyllum/in vivo culture/aseptic germination/proliferation/rooting分类
农业科技引用本文复制引用
罗盛娟,胡玉富,李晓君,曹琦,魏春梅..兜唇石斛离体快繁体系的建立[J].热带农业科学,2025,45(6):65-71,7.基金项目
滇西科技师范学院科研课题基金(No.DXXY202314) （No.DXXY202314）
临沧市科技局科技创新人才培养项目基金(No.202104AC100001-RC02). （No.202104AC100001-RC02）
