热带作物学报2025，Vol.46Issue(8)：1785-1794,10.DOI:10.3969/j.issn.1000-2561.2025.08.001
橡胶树HbMVD1基因启动子克隆及转录调控因子筛选
Cloning of the HbMVD1 Gene Promoter and Screening of Transcrip-tion Regulatory Factors in Rubber Tree
摘要
Abstract
Transcriptional regulation is one of the pivotal mechanisms governing biological activities.Natural rubber(NR),a crucial industrial raw material,is predominantly derived from rubber trees.The transcriptional regulation of NR biosynthesis has emerged as a focal point in the fundamental research of rubber tree in recent years.Mevalonate di-phosphate decarboxylase(MVD)is a key enzyme in the biosynthetic pathway of NR,yet its transcriptional regulation remains poorly understood.In this study,a 1500 bp promoter region of the HbMVD1 gene from rubber tree was cloned.Sequence analysis revealed the presence of various cis-acting elements,including the responsive to jasmonic acid,eth-ylene,mechanical wounding,light,and MYC binding.A gradient assay for autoactivation suppression demonstrated that the transcriptional autoactivation of the pAbAi-pHbMVD1 bait vector was effectively inhibited at an AbA concentration of 100 ng/mL.13 non-redundant proteins from a rubber tree latex yeast cDNA library that interact with the HbMVD1 promoter,including one WRKY transcription factor(HbWRKY1),one heat shock transcription factor,and one G-box binding factor were identified utilizing yeast one-hybrid screening.Tissue-specific expression analysis indicated that the genes encoding the proteins exhibited distinct expression patterns across different tissues.Transcriptional regulation analysis showed that HbWRKY1 activated the expression of HbMVD1 in vivo.Molecular docking revealed that HbWRKY 1 bound to the mechanical wounding element of the HbMVD1 promoter,and 11 potential amino acid binding sites were identified.This study would lay the groundwork for further elucidating the transcriptional regulatory network of HbMVD1 in rubber tree.关键词
橡胶树/HbMVD1启动子/酵母单杂交/转录因子/转录调控Key words
rubber tree/HbMVD1 promoter/yeast one-hybrid library/transcription factor/transcriptional regulation分类
农业科技引用本文复制引用
文连璇,杨署光,史敏晶,邓小敏,晁金泉..橡胶树HbMVD1基因启动子克隆及转录调控因子筛选[J].热带作物学报,2025,46(8):1785-1794,10.基金项目
海南省重点研发计划项目(No.ZDYF2022XDNY252) （No.ZDYF2022XDNY252）
现代农业产业技术体系建设专项(No.CARS-33-YZ1). （No.CARS-33-YZ1）
