热带作物学报2025，Vol.46Issue(8)：1795-1805,11.DOI:10.3969/j.issn.1000-2561.2025.08.002
木薯C3H基因鉴定及应答菜豆黄单胞菌侵染表达模式分析
Identification of Cassava C3H Genes and Analysis of the Expression Pattern in Response to Xanthomonas phaseoli pv.manihotis
摘要
Abstract
Cassava is one of the three major tuber crops in the world,and is also an important food crop in tropical and subtropical regions.Bacterial blight(CBB)is an important disease for cassava.CCCH(C3H)-type zinc finger proteins are widely present in most of organisms,not only involved in plant growth and development,hormone regulation,but also in response to biotic and abiotic stresses.Although the function of some C3H genes in other plants has been re-ported,but that in cassava has not been reported.In this study,we analyzed the phylogenetic characteristics,chromo-some location,gene structure,promoter cis-acting elements and collinearity of cassava C3H genes.A total of 89 C3H genes(MeC3H01-MeC3H89)were identified in cassava,which were distributed on 18 chromosomes.The phylogenetic tree showed that 89 MeC3H proteins were classified into 4 subfamilies.The C3H gene promoters contains multiple stress,hormones,plant growth and development response elements.39 pairs of cassava C3H genes had homologous relationships,indicating that these genes may be amplified by replication during evolution.We also analyzed the re-sponse expression pattern of C3H genes after cassava was infected by Xanthomonas phaseoli pv.manihotis(Xpm)by transcriptome and real-time quantitative PCR(qPCR)experiments.The expression levels of MeC3H49,MeC3H42 and MeC3H68 were significantly up-regulated,and those of MeC3H35,MeC3H77,MeC3H36,MeC3H31 and MeC3H86 were significantly down-regulated.In summary,we identified the cassava C3H gene family members and their expres-sion patterns in response to Xpm infection,which lays a foundation for further analysis of the function of MeC3Hs.关键词
木薯/C3H基因家族:基因表达:抗病响应:细菌性枯萎病Key words
cassava/C3H gene family/gene expression/disease resistance response/bacterial blight分类
农业科技引用本文复制引用
李敏,魏炳峥,贾素行,陈银华,李春霞..木薯C3H基因鉴定及应答菜豆黄单胞菌侵染表达模式分析[J].热带作物学报,2025,46(8):1795-1805,11.基金项目
海南省研究生创新课题(No.Qhys2023-250). （No.Qhys2023-250）
