水产学报2025，Vol.49Issue(8)：26-35,10.DOI:10.11964/jfc.20240414458
福建牡蛎二倍体和三倍体DNA甲基化分析
Analysis of DNA methylation in diploid and triploid of Crassostrea angulata
摘要
Abstract
DNA methylation is an important epigenetic mechanism.It can induce changes in the biological phenotype by alter-ing gene expression and the gene expression networks involved,thereby leading to the process of adaptive evolution.At present,epigenetic studies of polyploid genomes have been carried out in multiple aquatic animals,but epigenetic research on the polyploid genome of Crassostrea angulata has not been reported yet.In order to explore the methylation status of genomic DNA in different tissues of diploid and triploid C.angulata,the experiment used the fluorescence-labeled methylation sensit-ive amplified polymorphism(F-MSAP)technology to compare and analyze genomic DNA methylation in four tissues of C.angulata(mantle,gill,gonad,and adductor).The results showed that a total of 1 388 to 3 871 methylation sensitive sites were detected.The total methylation levels of the mantle,gill,gonad,and adductor muscle in diploid and triploid populations were 21.05%and 21.73%,20.44%and 18.65%,22.04%and 22.55%,and 21.12%and 33.56%,respectively.There were extremely significant epigenetic differences among the tissues.The full methylation levels of diploids ranged from 11.75%to 12.84%,while the hemimethylation levels ranged from 7.60%to 9.82%,and the total methylation levels ranged from 20.44%to 22.04%.The full methylation levels of triploids ranged from 11.26%to 30.28%,while the hemimethylation levels ranged from 3.28%to 10.47%,and the total methylation levels ranged from 18.65%to 33.56%.The total methylation level of triploids increased by 0.51%-12.44%compared with diploids.Full methylation was the predominant methylation mode,and the epigenetic differ-ences between diploids and triploids were extremely significant.The research shows that the mantle,gill,gonad,and adductor muscle tissues of C.angulata have different degrees of methylation levels.Methylation changes have occurred in triploid C.angulata,and these changes may affect the way of gene expression.This study can provide reference materials for the epigen-etic research of polyploid C.angulata.关键词
福建牡蛎/二倍体/三倍体/DNA甲基化/荧光标记的甲基化敏感扩增多态性(F-MSAP)Key words
Crassostrea angulata/diploid/triploid/DNA methylation/fluorescence-labeled methylation sensitive amplified polymorphism(F-MSAP)分类
生物科学引用本文复制引用
陆莉莉,徐成勋,孔令锋,李琪..福建牡蛎二倍体和三倍体DNA甲基化分析[J].水产学报,2025,49(8):26-35,10.基金项目
山东省农业良种工程项目(2022LZGCQY010) （2022LZGCQY010）
山东省重点研发计划(2022TZXD002) Agriculture Seed Improvement Project of Shandong Province(2022LZGCQY010) （2022TZXD002）
Key Research and Development Program of Shandong Province(2022TZXD002) （2022TZXD002）
