水产学报2025，Vol.49Issue(8)：36-47,12.DOI:10.11964/jfc.20240714616
温度对美洲鲥胚胎发育的影响
Effect of temperature on the embryonic development of American shad(Alosa sapidissima)
摘要
Abstract
Temperature is a significant stressor affecting the growth and development of American shad(Alosa sapidissima).This study aims to investigate the effects of different hatching temperatures on the embryonic development and larval hatching of A.sapidissima.Five hatching temperatures were set at 16,18,20,22 and 24℃.The embryonic development and larval sur-vival rates were recorded,and the gene expression levels at different embryonic development stages were analyzed at the hatch-ing temperature of 18℃.The results showed that complete embryonic development was observed at hatching temperatures of 16,18,20 and 22℃.As the hatching temperature increased,the total hatching duration decreased.The total duration from fer-tilization to larval hatching was 103 h 50 min,84 h 40 min,78 h 40 min,and 64 h 50 min at hatching temperatures of 16,18,20,and 22℃,respectively.At hatching temperatures of 18℃and 20℃,the hatching rate and larval survival rate significantly increased(P<0.05).At a hatching temperature of 22℃,the embryonic mortality rate was significantly higher than that at 16,18,and 20℃(P<0.05).At a hatching temperature of 18℃,the genes hif1α,hspb1,fbf1,hec,asgr1 and vegfα were inhibited dur-ing the cell proliferation period but were significantly activated during the cell differentiation period(P<0.05).During the hatching and pre-hatching organ formation periods,hec expression was significantly activated(P<0.05).In the early embryonic development stage,sod and gst were highly expressed(P<0.05),while hspb1 and hif1α were highly expressed in the late organ formation and hatching stages(P<0.05).Rbpj was highly expressed during the multi-cellular and blastocyst stages of early embryonic development(P<0.05)and downregulated as embryonic development progressed(P<0.05).The optimal hatching temperature for A.sapidissima is 18-20℃.The blastula stage is a critical period in the embryonic development of A.sapi-dissima,in which the expression of genes related to cell formation and division starts to be downregulated,and the expression of genes related to organ formation and cell differentiation is significantly upregulated.This study provides a reference for the early development regulation,seed production and selective breeding of A.sapidissima.关键词
美洲鲥/温度/胚胎发育/基因表达/遗传育种Key words
Alosa sapidissima/temperature/embryonic development/gene expression/genetic breeding分类
农业科技引用本文复制引用
梁政远,蒋超,解旭东,董在杰,缪凌鸿,刘影,罗明坤,冯冰冰,朱文彬,龚雅婷,史秀兰,李玉林..温度对美洲鲥胚胎发育的影响[J].水产学报,2025,49(8):36-47,12.基金项目
江苏省种业振兴揭榜挂帅项目(JBGS[2021]131) （JBGS[2021]131）
江苏省农业产业技术体系(JATS[2021]367) Seed Industry Revitalization Project of Jiangsu Province(JBGS[2021]131) （JATS[2021]367）
Jiangsu Agricultural Industry Technology System(JATS[2021]367) （JATS[2021]367）
