水产学报2025，Vol.49Issue(8)：48-58,11.DOI:10.11964/jfc.20220513510
水体中低浓度溴氰菊酯慢性胁迫对中华绒螯蟹肠道组织及微生物群的影响
Chronic potential of low concentration aqueous deltamethrin to intestinal histology and gut microbiota in Eriocheir sinensis
摘要
Abstract
Deltamethrin(DM)has been used in aquaculture as pests defender,parasites remover,salmon delouser,ponds cleanser,and so on.The low-dose DM,less than safety concentration(SC)in surface water is ubiquitous and lasts for the whole agricultural period because of frequent use.To understand the chronic stress of deltamethrin below SC on Eriocheir sinensis intestine,this research acquired the SC as 0.190 μg/L calculated from the data of acute experiments with Turubell formula,and then investigated on juvenile E.sinensis exposed to 0,0.024,0.095 and 0.380 μg/L DM for 30 days.Two samples were taken from each level at 0 d and 30 d,and examined intestinal histological structures on the slices stained with H.E.and also ana-lyzed gut microbiota based on the sequence(after DNA extraction,amplification and library construction).The main results showed that ①Histological examination showed DM damaged the intestine histological structure of juvenile E.sinensis,whose adverse effects increased with the increment of DM concentration.Exposed to DM of 0.380 μg/L,the mucosal epithelial cells were collapsed,in which the mucous layer separated from the submucous layer;the villi were destructed,in which the cuticle layer separated from the mucous layer,even intestinal glands messed up.Exposed to DM of 0.095 μg/L,some part of the mucous layers were collapsed,mucous layer cells were partly degraded,and intestinal glands were partly messed up.Exposed to both 0.024 and 0 μg/L DM,there were no observable effects.②The gut microbiota equilibrium was broken in the intestine of E.sinensis exposed to DM of chronic concentration.Ace and Chao1 indexes decreased with the increment of DM concentration,which indicated DM decreased the microbial diversity in intestine ecology.③In phylum-level analysis,the beneficial bacteria was decreased and pathogenic bacteria was increased in the intestinal microbiota of juvenile E.sinensis exposed to DM below the SC.DM increased the relative abundances of Proteobacteria in 0.380 μg/L treatment(P<0.01),and Bacteroidota in 0.095 μg/L treatment(P<0.05),decreased the relative abundances of Tenericutes in 0.095 and 0.380 μg/L treat-ments(P<0.05),and Firmicutes in 0.024,0.095 and 0.380 μg/L treatments(P<0.05).④In genus-level analysis,DM increased the relative abundances of Acinetobacter,Bacteroides,and Wolbachia in all treatments(P<0.05),even resulted in some patho-genic bacteria of Pseudomonas,Shewanella,Chryseobacterium,and Flavobacterium in 0.380 μg/L treatment.In summary,DM of SC caused E.sinensis intestine in chronic stress,which damaged the intestine histological structure,broke the equilibrium of beneficial microbiota,and increased the relative abundances of pathogenic bacteria.The calculated safety concentration is not truly safe,which indicates that real DM safety concentration for juvenile E.sinensisneeds more trials in aquaculture.关键词
中华绒螯蟹/溴氰菊酯/肠道健康/安全浓度Key words
Eriocheir sinensis/deltamethrin/intestinal health/safety concentration分类
资源环境引用本文复制引用
石今朝,陶贤继,姜晓东,成永旭,吴旭干..水体中低浓度溴氰菊酯慢性胁迫对中华绒螯蟹肠道组织及微生物群的影响[J].水产学报,2025,49(8):48-58,11.基金项目
现代农业产业技术体系(CARS-48) （CARS-48）
外交部亚洲合作基 金(125A0607) （125A0607）
江苏省种业振兴"揭榜挂帅"项目(JBGS[2021]127) （JBGS[2021]127）
上海市水产动物良种创制与绿色养殖协同创新中心项目(2021科技02-12) Chinese Agriculture Research System(CARS-48) （2021科技02-12）
Ministry of Foreign Affairs Cooperation Funds Projects in Asia(125A0607) （125A0607）
the"JBGS"Project of Seed Industry Revitalization in Jiangsu Province(JBGS[2021]127) （JBGS[2021]127）
Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals(2021-KJ-02-12) （2021-KJ-02-12）
