山东科学2025，Vol.38Issue(4)：39-45,7.DOI:10.3976/j.issn.1002-4026.20240097
领鞭毛虫Salpingoeca rosetta的纤毛组装研究
Research on the cilia assembly in Salpingoeca rosetta
摘要
Abstract
To investigate the mechanism of the cilia assembly in choanoflagellates,this study focused on a colony-forming choanoflagellate,Salpingoeca rosetta.For the first time,the cilia of Salpingoeca rosetta were disassembled viamechanical shearing,and a LiCl(lithium chloride)-induced ciliaelongation experiment was conducted to investigate mechanisms underlying the disassembly,regeneration,and assembly of the cilia of Salpingoeca rosetta.In the LiCl-induced ciliaelongation experiment,a final molarity of 250 mmol/L was used.In the cilia disassembly and regeneration experiments,the mechanical shearing conditions were determined.Experimental results show that the cilia of Salpingoeca rosetta were restored to their original lengths after a mechanical force was applied on them for 120 min;moreover,LiCl-induced a ciliaelongation by more than 60%after 180 min of LiCl treatment.Compared with the cold-stress-induced disassembly of the cilia of Salpingoeca rosetta,the mechanical shearing method was faster,more efficient,and simpler with higher repeatability.This study provides a fundamental method for investigating the molecular mechanism of the cilia assembly in Salpingoeca rosetta.关键词
领鞭毛虫/氯化锂诱导纤毛延伸/纤毛再生/纤毛组装/机械力切割Key words
Salpingoeca rosetta/LiCl-induced cilia elongation/cilia regeneration/cilia assembly/mechanical shearing分类
生物科学引用本文复制引用
吴倩龙,胡昆,胡哲文,张颖,田爱洁,冯雳..领鞭毛虫Salpingoeca rosetta的纤毛组装研究[J].山东科学,2025,38(4):39-45,7.基金项目
国家重点研发计划(2020YFA0907400) （2020YFA0907400）
国家自然科学基金项目(32170702) （32170702）
