山东科学2025，Vol.38Issue(4)：46-55,10.DOI:10.3976/j.issn.1002-4026.20240078
鱼腥草白绢病病原菌的分离鉴定及其生防木霉的筛选
The isolation and identification of the southern blight-causing pathogen in Houttuynia cordata Thunb.and screening of its biocontrol agent Trichoderma spp
摘要
Abstract
Southern blight is a key disease that harms the growth of Houttuynia cordata Thunb.To identify the pathogenic fungi that cause southern blight in H.cordata,tissue isolation was conducted on H.cordata samples infected with southern blight from Dangyang,Hubei province,China.Furthermore,screening was conducted to determine the morphology and molecular biology of the infected plant,and the isolated pathogens were screened against the antagonist Trichoderma.The results identified Athelia rolfsii as isolated pathogenic fungi.Three Trichoderma strains with fungal inhibition rate greater than 95%were screened through a plate confrontation assay.Among them,Trichoderma asperellum QT21918 exhibited the effects of hyphal hyperparasitism and fungal lysis on A.rolfsii and its inhibitory rates for volatile and nonvolatile metabolites were 38.21%and 50.54%,respectively.In vitro leaf tests demonstrated that this strain had a fungal inhibitory rate of 67.7%against the spread of the lesions associated with southern blight disease.A pot-based experiment showed that optimal control effect was achieved by simultaneously inoculating T.asperellum QT 21918 and the pathogen as well as by first inoculating T.asperellum QT21918 for 7 days and then inoculating the pathogen,both methods achieving a control effect of 100%.The above results indicate that T.asperellum QT21918 has potential as a biocontrol agent against southern blight in H.cordata.关键词
鱼腥草/病原菌/生物防治/木霉Key words
Houttuynia cordata/pathogenic fungi/biological control/Trichoderma分类
农业科技引用本文复制引用
王兴强,李红梅,徐维生,魏艳丽,陈凯,李纪顺..鱼腥草白绢病病原菌的分离鉴定及其生防木霉的筛选[J].山东科学,2025,38(4):46-55,10.基金项目
山东省科学院科教产融合试点工程国际合作项目(2022GH011、2022GH012) （2022GH011、2022GH012）
济南市高校"20条"(自主培养创新团队)项目(2020GXRC003) （自主培养创新团队）
