山东科学2025，Vol.38Issue(4)：56-66,11.DOI:10.3976/j.issn.1002-4026.20240087
大姜根腐病生防木霉菌株的分离与鉴定
Isolation and identification of Trichoderma strains against ginger root rot
摘要
Abstract
Ginger root rot is a soil-borne disease in ginger planting,which is mainly caused by Fusarium spp.and can induce serious reduction or even extinction of ginger yield.Thirty-four Trichoderma strains with antifungal activity were isolated from the soil collected from representative ginger planting areas in Shandong province.Based on their antibiotic properties,antagonistic coefficients,and spore production capabilities,three highly effective Trichoderma strains were selected for their antagonism against Fusarium oxysporum f.sp.zingiberi(Foz):TW20111,TW20321,and TW20323.Morphological observations and molecular biological identifications classified strain TW20111 as Trichoderma atroviride and strains TW20321 and TW20323 as Trichoderma harzianum.The efficacy of these three Trichoderma strains in controlling ginger root rot was assessed under greenhouse and field conditions.Under greenhouse conditions,T.harzianum TW20321 showed the highest control efficacy of 86.33％,significantly promoted ginger plant height,and moderately increased ginger yield compared to carbendazim treatment.Field experiments revealed that the control efficacy of any two combined Trichoderma strains was significantly higher than that of a single strain,with the combination of TW20111 and TW20321 achieving the highest control efficacy of 68.90％.This combination also resulted in the greatest increases in plant height and single plant yield,which rose by 19.31％and 27.43％,respectively,compared to the control group.This study provided a basis for the development of new bio-pesticides for the effective control of ginger root rot.关键词
木霉/大姜根腐病/尖孢镰刀菌大姜专化型/生物防治Key words
Trichoderma spp./ginger root rot/Fusarium oxysporum f.sp.zingiberi/biocontrol分类
农业科技引用本文复制引用
蒋艳青,杨建闻,李杨,孙海森,扈进冬,李纪顺,杨合同,吴远征..大姜根腐病生防木霉菌株的分离与鉴定[J].山东科学,2025,38(4):56-66,11.基金项目
山东省科技型中小企业创新能力提升工程项目(2022TSGC2370) （2022TSGC2370）
山东省自然科学基金项目(ZR2021MC085) （ZR2021MC085）
齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(国合专项)(2024GH15) （山东省科学院）
