山东科学2025，Vol.38Issue(4)：78-85,8.DOI:10.3976/j.issn.1002-4026.20240083
高产油小球藻的超声波诱变育种
Ultrasound-induced mutation breeding of high lipid content Chlorella sp
摘要
Abstract
To enhance the microalgae lipid content,Chlorella sp.was subjected to ultrasonic mutagenesis.Mutagenesis was performed for 20 minutes under conditions of 40 kHz and 100 W,resulting in the cultivation of 10 mutant strains(M1-M10).Further screening led to the selection of three stable high-lipid-producing strains,namely M2,M5,and M7.These strains were analyzed to determine their biomass concentration and lipid,chlorophyll,and protein mass concentration and percentage.The results indicated that the biomass mass concentrations of M2,M5,and M7 were 0.384,0.379,and 0.443 g/L,respectively.The lipid yields of M2,M5,and M7 were 14.70,13.34,and 25.11 mg/L,respectively,all of which were higher than that of the wild type(WT)strain,which had a recorded yield of 9.38 mg/L.Compared to the WT,the chlorophyll yield of the mutant strains increased by 14.41%,3.01%,and 10.24%,respectively,while the protein content of the mutant strains increased by 7.30%,10.52%,and 13.66%,respectively.This study is of great significance to the industrialized production of biodiesel.关键词
小球藻/超声波/生物量/油脂/叶绿素/蛋白质Key words
Chlorella sp./ultrasound/biomass/lipid/chlorophyll/protein分类
生物科学引用本文复制引用
宋刘洋,宋文路,睢站斌,张琪,王金国..高产油小球藻的超声波诱变育种[J].山东科学,2025,38(4):78-85,8.基金项目
国家级大学生研究性学习和创新性实验计划项目(202110454003) （202110454003）
