药学研究2025，Vol.44Issue(6)：536-542,7.DOI:10.13506/j.cnki.jpr.2025.06.003
芭蕉根安神方对小鼠睡眠的改善作用及机制分析
Effect and mechanism of Musa basjoo Anshen formula on sleep improvement in mice
摘要
Abstract
Objective To study the sedative effect and mechanism of Musa basjoo Anshen formula on sleep improve-ment in mice.Methods Normal mice,synergistic subthreshold,and sleep dose of Urethane were used to observe the effects of Musa basjoo Anshen formula on spontaneous activity,sleep rate,sleep latency,and sleep duration on mice;The PCPA-in-duced insomnia mouse model was tested by elevated cross maze test and open field test,and the contents of 5-HT,GABA and GLU in the brain tissue were detected.The protein expression levels of HTR1A,GABRG2 and GABRA1 were deter-mined by Western blotting technique.Results Musa basjoo Anshen formula had no significant effect on the autonomic activ-ity of normal mice(P＞0.05).The number of sleeping animals and sleeping rate of mice with subthreshold dose of Urethane were significantly increased(P＜0.05).The number of open arms and the percentage of stay time of PCPA-induced insomnia mice entering the elevated cross maze were significantly increased(P＜0.05,P＜0.01).The upright times of PCPA-induced insomnia mice in open field were significantly increased(P＜0.01),and the modification times were significantly decreased(P＜0.05).The results showed that Musa basjoo Anshen formula could significantly increase the contents of 5-HT and GA-BA in the brain tissues of PCPA-induced insomnia mice(P＜0.05),and significantly decrease the content of GLU in the brain tissues of PCPA-induced insomnia mice(P＜0.05).Western blotting detection showed that the protein expression of HTR1A,GABRG2 and GABRA1 could be significantly increased by Musa basjoo Anshen formula(P＜0.05).Conclusion Musa basjoo Anshen formula can regulate the expression of sleep-related HTR1A,GABRG2 and GABRA1 proteins,improve sleep and anxiety in mice,and have sedative and tranquilizing effects.关键词
芭蕉根安神方/镇静安神/对氯苯丙氨酸/睡眠/焦虑Key words
Musa basjoo Anshen formula/Sedation and tranquilization/p-chlorophenylalanine/Sleep/Anxiety分类
医药卫生引用本文复制引用
唐慧勤,陆益平,刘远洋,李玉强,郭巧琴,曾春晖,章昱,林群峰,杨柯..芭蕉根安神方对小鼠睡眠的改善作用及机制分析[J].药学研究,2025,44(6):536-542,7.基金项目
国家中医药管理局"中药炮制传承技术传承基地"资助项目(No.桂中医药发[2022]9号) （No.桂中医药发[2022]9号）
校企合作项目(No.H2023007JX) （No.H2023007JX）
