药学研究2025，Vol.44Issue(7)：649-654,673,7.DOI:10.13506/j.cnki.jpr.2025.07.004
基于UPLC-QTOF-MS的白花蛇舌草醇提物成分表征及其抗肝癌谱效关系
Characterization of the compounds of Hedyotis diffusa alcohol extract based on UPLC-QTOF-MS and its spectral effect relationship of liver cancer
摘要
Abstract
Objective To elucidate the substance basis of Hedyotis diffusa alcohol extract for anti liver cancer through UPLC-QTOF-MS technology and spectral correlation analysis.Methods HPLC was used to establish ten batches of fingerprint spectra of Hedyotis diffusa alcohol extract,and the anti liver cancer efficacy of Hedyotis diffusa alcohol extract was analyzed using the proliferation inhibition rate and apoptosis necrosis rate of liver cancer cell line SMMC-7721 as indicators.Further analyze the spectrum effect correlation of Hedyotis diffusa alcohol extract against liver cancer using grey relational analysis and partial least squares analysis.Finally,UPLC-QTOF-MS technology was used to identify the common peaks.Results Hedyotis diffusa alcohol extract significantly inhibited the proliferation and induced apoptosis of liver cancer cell line SMMC-7721,with an IC50 of 3.42 mg·mL-1.Spectral analysis shows that peaks 12,4,44,9,11,7,and 10 are positively correlated,while peaks 2,38,27,and 29 are negatively correlated.Among them,peak 12 is 5-caffeoylquinic acid,peak 4 is 4-OD-p-glucopyranosyl-p-coumaric acid,peak 9 is tachiogroside B,and peak 2 is succinic acid.Conclusion The Hedyotis diffusa alcohol extract could inhibit the proliferation of liver cancer cells,and its material basis may be 5-caffeoylquinic acid,4-OD-glucopyranosyl-p-coumaric acid,tachiogroside B,etc.关键词
白花蛇舌草/谱效相关/肝癌/物质基础/超高效液相色谱四极杆飞行时间串联质谱法Key words
Hedyotis diffusa/Spectral efficiency correlation/Liver cancer/Material basis/UPLC-QTOF-MS分类
医药卫生引用本文复制引用
于家欣,张强,孟宪生..基于UPLC-QTOF-MS的白花蛇舌草醇提物成分表征及其抗肝癌谱效关系[J].药学研究,2025,44(7):649-654,673,7.基金项目
辽宁省中医药现代化研究创新团队项目(No.LT2017015) （No.LT2017015）
2024年度辽宁省教育厅高校基本科研项目储备项目-优秀研究生项目(No.2024-JYTCB-043) （No.2024-JYTCB-043）
