山东农业科学2025，Vol.57Issue(7)：10-18,9.DOI:10.14083/j.issn.1001-4942.2025.07.002
高粱GAox基因家族的鉴定及其在种子萌发中的表达分析
Identification of Sorghum GAox Gene Family and their Expression Analysis during Seed Germination
摘要
Abstract
The GAox family are the key enzymes in the gibberellin synthesis pathway.This study conduc-ted identification and bioinformatics analysis of the GAox family in sorghum(Sorghum bicolor L.)and valida-ted the expression of GAox genes during seed germination using qRT-PCR.The results showed that a total of 16 GAox gene family members were identified,and they unevenly distributed on 6 chromosomes and encoded 323 to 498 amino acids.These GAox proteins were hydrophilic proteins located in the cytoplasm with isoelectric point as 4.80 to 9.58,instability index as 36.17 to 54.87,and fat coefficient as 69.29 to 90.81.Through phy-logenetic analysis,it was found that the GAox gene family could be divided into four subfamilies as GA20ox,GA3ox,C20GA2ox and C19GA2ox,which contained 4,2,3 and 7 members,respectively.The intra-and in-ter-group collinearity analysis of GAox genes showed that 5 pairs of duplicated genes were identified in sor-ghum,and 29 pairs of collinearity genes were found between sorghum and rice.Through the analysis of gene structure and conserved motifs,it was found that the sorghum GAox gene family members contained 1 to 4 ex-ons,0 to 3 introns and 15 conserved motifs.All the 16 sorghum GAox members contained abscisic acid re-sponse elements,jasmonic acid methyl ester response elements and light response elements,suggesting their functions related to plant growth and development,light response and plant hormones.Further analysis of ex-pression patterns showed that the expression of sorghum GAox genes was tissue-specific,suggesting different functions in sorghum growth and development.The qRT-PCR analysis revealed that the 16 genes were upregu-lated during seed germination,but the expression peak occurred at different time,which suggested that differ-ent GAox genes might play roles at different germination stages of sorghum seeds.These results could provide a theoretical basis for subsequent functional research of the sorghum GAox genes.关键词
高粱/GAox基因家族/生物信息学分析/种子萌发/表达模式Key words
Sorghum bicolor L./GAox gene family/Bioinformatics analysis/Seed germination/Expres-sion pattern分类
农业科技引用本文复制引用
姜昱雯,任艳,陈满静,赵强,张国兵,沈佳奇,高旭,邵明波..高粱GAox基因家族的鉴定及其在种子萌发中的表达分析[J].山东农业科学,2025,57(7):10-18,9.基金项目
贵州省农业科学院青年科技基金项目(黔农科一般基金[2025]21号,黔农科一般基金[2024]09号) （黔农科一般基金[2025]21号,黔农科一般基金[2024]09号）
贵州省特色杂粮生物育种全省重点实验室项目(黔科合平台ZSYS[2025]026) （黔科合平台ZSYS[2025]026）
贵州省科技计划项目(黔科合基础-ZK[2023]一般169) （黔科合基础-ZK[2023]一般169）
中央引导地方科技发展基金项目(黔科中引地[2022]4045) （黔科中引地[2022]4045）
遵义市科技与大数据、茅台学院市校联合科技合作资金项目(遵义市科合HZ字[2024]373号) （遵义市科合HZ字[2024]373号）
