山东农业科学2025，Vol.57Issue(7)：26-35,10.DOI:10.14083/j.issn.1001-4942.2025.07.004
君迁子DlWRKY3基因的克隆及其对逆境胁迫的响应
Cloning and Expression Analysis of DlWRKY3 Gene in Diospyros lotus under Abiotic Stresses
摘要
Abstract
In order to study the specific role of DlWRKY3 gene in response to abiotic stresses,the nucle-otide sequence of DlWRKY3 gene was cloned using homologous cloning method with the nucleotide sequence of DkWRKY3 gene of Diospyros kaki as reference.Bioinformatics and expression analyses of DlWRKY3 gene were performed.The results showed that the length of open reading frame(ORF)of DlWRKY3 gene was 951 bp,encoding 316 amino acids.The molecular formula of DlWRKY3 protein was C1490H2401N447O490S15,and the the-oretical isoelectric point was 8.52.It was an unstable alkaline hydrophilic protein with not transmembrane do-main or signal peptide region.The predicted secondary structure of this protein was dominated by α-helix and random curling.It was a type Ⅱ WRKY transcription factor with the typical WRKYGQK domain and a C2H2 zinc finger structure.Homologous comparison showed that this gene was closely related to Arabidopsis At-WRKY40 and cotton GhWRKY3.The results of fluorescence quantitative PCR indicated that the expression of DlWRKY3 was induced by low temperature stress,and inhibited by drought and salt stresses.In this study,Dl-WRKY3 gene was successfully cloned,which laid a foundation for further study on the role of DlWRKY3 gene in response to abiotic stresses,and provided the important gene resource and theoretical basis for stress-resist-ant molecular breeding of Diospyros plants.关键词
君迁子/DlWRKY3/基因克隆/生物信息学分析/逆境胁迫Key words
Diospyros lotus L./DlWRKY3/Gene cloning/Bioinformatics analysis/Abiotic stresses分类
农业科技引用本文复制引用
张晓娜,闫澳阳,索得锟,周瑞金,侯小进,扈惠灵..君迁子DlWRKY3基因的克隆及其对逆境胁迫的响应[J].山东农业科学,2025,57(7):26-35,10.基金项目
河南省重点研发与推广专项(科技攻关)项目"DlWRKY3转录因子在柿逆境胁迫应答中的调节作用及柿抗逆新种质创制"(222102110368) （科技攻关）
