山东农业科学2025，Vol.57Issue(7)：36-43,8.DOI:10.14083/j.issn.1001-4942.2025.07.005
基于转录组测序鉴定油莎豆油脂合成关键基因的研究
Identification of Key Genes Related to Cyperus esculentus Lipid Synthesis Based on Transcriptome Sequencing
摘要
Abstract
Cyperus esculentus L.is a new type of oilseed crop,whose underground tubers are rich in lip-id with high oleic acid,so it is of high developmental value.In this study,1 465 significantly differentially ex-pressed genes were screened out based on the difference in lipid accumulation by comparative analysis of tran-scriptome data from tubers at different developmental stages.Among which,676 up-regulated genes were sig-nificantly enriched in fatty acid biosynthesis,sucrose metabolism,glycolysis and other related metabolic path-ways,and the contribution of fatty acid biosynthesis pathway was much higher than that of triglyceride(TAG)biosynthesis pathway.The lipid synthesis core enzyme genes of AC Case,FATA,SAD and DGAT,and the key regulatory genes for oil-body forming proteins Caleosin and Oleosin were identified.These results would provide a theoretical basis for exploring the molecular mechanism of lipid synthesis of C.esculentus.关键词
油莎豆/转录组测序/油脂合成/候选基因鉴定Key words
Cyperus esculentus L./Transcriptome sequencing/Lipid synthesis/Identification of candidate genes分类
农业科技引用本文复制引用
高云飞,张君,丛炜轩,杨松楠,张健,王真慧,曹珊珊,李雪莹..基于转录组测序鉴定油莎豆油脂合成关键基因的研究[J].山东农业科学,2025,57(7):36-43,8.基金项目
吉林省发展和改革委员会2023年省预算内基本建设(创新能力建设)-产业技术研究与开发项目"高产、优质油莎豆种质创新与新品种选育"(2023C035-5) （创新能力建设）
