实用医学杂志2025，Vol.41Issue(14)：2174-2182,9.DOI:10.3969/j.issn.1006-5725.2025.14.008
LCN2介导EGFR磷酸化对人输卵管上皮细胞炎性损伤的影响
The effect of LCN2-mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epi-thelial cells
摘要
Abstract
Objective To investigate the regulatory effect of lipocalin 2(LCN2)on epidermal growth factor receptor(EGFR)phosphorylation and its impact on inflammatory damage in human fallopian tube epithelial cells.Methods Human fallopian tube epithelial cells were isolated and a lipopolysaccharide(LPS)intervention was applied to establish an in vitro cell model.The cells were randomly assigned into the following groups:a blank control group(Control),a model group(Model),experimental groups(Model+si-LCN2 or Model+oe-LCN2),and negative control groups(Model+si-NC or Model+oe-NC).Changes in cell viability,apoptosis rates,inflam-matory levels,as well as the expression of EGFR mRNA,LCN2,EGFR,p-EGFR,and the ratio of p-EGFR/EGFR proteins were evaluated.Results Compared to the Model group,the Model+si-LCN2 group exhibited enhanced cell viability,a reduced apoptosis rate,and decreased expression of inflammatory factors(P＜0.05).Immunopre-cipitation analysis confirmed a direct interaction between LCN2 and EGFR.In comparison with the Model group,the Model+oe-LCN2 group demonstrated elevated levels of p-EGFR and the p-EGFR/EGFR ratio(P＜0.05),while no significant change was observed in total EGFR expression(P＞0.05).Conclusion Inhibition of LCN2-mediated EGFR phosphorylation enhances cell viability,reduces apoptosis,and mitigates inflammatory responses,thereby ameliorating LPS-induced inflammatory injury in human fallopian tube epithelial cells.关键词
脂钙蛋白2/人输卵管上皮细胞炎性损伤/表皮生长因子受体/表皮生长因子受体磷酸化Key words
LCN2/inflammatory damage of human fallopian tube epithelial cells/EGFR/EGFR phosphorylation分类
医药卫生引用本文复制引用
夏亚琼,朱悦,蒋呈呈,谭鸿平,张雁,刘玲..LCN2介导EGFR磷酸化对人输卵管上皮细胞炎性损伤的影响[J].实用医学杂志,2025,41(14):2174-2182,9.基金项目
国家自然科学基金项目(编号:81804140) （编号:81804140）
湖南省卫生科研课题(编号:20232627) （编号:20232627）
湖南省2022年度"神农人才"工程学科带头人——青年神农学者项目(编号:湘中医药[2022]4号) （编号:湘中医药[2022]4号）
2022年度湖湘青年科技创新人才项目 （）
