天津农业科学2025，Vol.31Issue(6)：7-13,7.DOI:10.3969/j.issn.1006-6500.2025.06.002
秀丽四照花组培快繁体系建立
Establishment of Rapid Multiplication System for Tissue Culture of Cornus hongkongensis subsp.elegans
摘要
Abstract
In order to establish a rapid propagation system for Cornus florida,tissue culture methods were used.The study involved screening sterilization methods for stem segment explants of Cornus florida and investigating the effects of different concentrations of hormones NAA and 6-BA on callus induction,bud differentiation,young leaf proliferation,and rooting.The results indicated that ster-ilization with 0.1％HgCl2 for 12 minutes was optimal.Callus induction was most effective with MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,reaching a 90％induction rate after 40 days.Bud differentiation from callus was best promoted by MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA.Shoot proliferation from sterile young leaves was highest with MS+1.0 mg·L-1 NAA+0.5 mg·L-1 6-BA.Root induction was best in MS+0.5 mg·L-1 IBA,however,the induced roots were prone to browning,necessitating timely subculture onto a rejuvena-tion medium In condusion,this study provides a technical foundation for the large-scale propagation of this species,which is of great significance for its resource development and industrial application.关键词
秀丽四照花/组织培养/茎段/愈伤组织Key words
Cornus hongkongensis subsp.elegans/tissue culture/stem segment/callus分类
农业科技引用本文复制引用
阮淑明,赖士淦,周显臻..秀丽四照花组培快繁体系建立[J].天津农业科学,2025,31(6):7-13,7.基金项目
福建省林业科技资助项目(闽林科便函[2020]29号) （闽林科便函[2020]29号）
