西安科技大学学报2025，Vol.45Issue(4)：704-715,743,13.DOI:10.13800/j.cnki.xakjdxxb.2025.0407
利用生物方法代谢煤矿采空区CO气体的生物降解特性
Biodegradation characteristics of CO gas in coal mine goaf using biological methods
摘要
Abstract
Underground CO overrun threatens coal mine safety production as well as coal spontaneous combustion prediction and warning.In this paper,a biological treatment method was used to extract and purify colonies from samples from Chenjiashan and Xiashijie coal mines,to screen to obtain three single strains of bacteria with high CO degradation activity,namely Pseudomonas,Chryseobacterium,and Co-mamonas,and to investigate the adaptability of the three single strains of bacteria to different cultivation environments.The degradation characteristics of the three single strains of bacteria were analyzed by successive passage cultures in a dominant environment.The results show that:The three strains of car-bon monoxide oxidizing monoclonal bacteria grow well in the temperature range of 25～40℃ and pH 6.0～9.0,but are strongly affected by the acidic and low-temperature environments.Furthermore,when the initial concentration of CO gas is 21.63％,the CO gas metabolism rates of three strains of bacteria can reach 98.52％,98.59％,and 97.80％,respectively.As the concentration of CO gas increases,the metabolism rates show a gradual decline,which may be related to factors such as atmospheric pressure and oxygen concentration in the culture environment.Optimizing culture conditions for continuous pas-sage of microorganisms can enhance the growth activity of carbon monoxide-oxidizing bacteria,increase their growth concentration,and accelerate the CO gas metabolism rate.There is a good linear relation-ship between growth concentration and the variation in gas carbon source metabolism rates.The carbon monoxide oxidizing bacteria obtained from the study have strong environmental adaptability and high metabolic capacity,and have significant theoretical significance in eliminating the toxic and harmful effects of CO gas in environments such as mines,ensuring safe and green mining in coal mines.关键词
煤矿/一氧化碳氧化菌/CO气体/生长代谢特性/降解特性Key words
coal mine/carbon monoxide oxidizing bacteria/CO gas/growth and metabolic properties/degradation properties分类
矿业与冶金引用本文复制引用
王彩萍,张宇,邓寅,段夏丹,王津睿,王宁,郑思雨..利用生物方法代谢煤矿采空区CO气体的生物降解特性[J].西安科技大学学报,2025,45(4):704-715,743,13.基金项目
国家自然科学基金项目(52174201,52474253) （52174201,52474253）
陕西省杰出青年科学基金项目(2022JC-30) （2022JC-30）
四川省青年基金项目(2025ZNSFSC1362) （2025ZNSFSC1362）
