新发传染病电子杂志2025，Vol.10Issue(3)：1-5,5.DOI:10.19871/j.cnki.xfcrbzz.2025.03.001
深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究
Surveillance of Chlamydia psittaci in a bird market in Shenzhen and genomic characterization of isolated strains
摘要
Abstract
Objective To screen for the presence of Chlamydia psittaci(Cps)in parrots from bird and flower markets in Shenzhen,and to conduct a comprehensive analysis of the positive samples,providing a scientific basis for epidemiological research,disease prevention and control,and public health management.Method In May 2023 and June 2024,two rounds of investigation and sampling were conducted at a parrot retail stall in a wholesale agricultural market in Shenzhen.The collected samples were screened for Cps,and Cps-positive samples were subjected to isolation through cell culture.Whole-genome sequencing was performed on the isolated Cps strains,followed by genomic analysis to explore genotype distribution and evolutionary relationships.Result A total of 110 samples were collected,of which 38 tested positive for Cps,with a positivity rate of 34.5%(38/110).One Cps strain was successfully isolated from the intestinal mucosa of a Cps-positive parrot.Whole-genome sequencing yielded the complete genome of the isolate,which was identified as genotype A.Phylogenetic analysis revealed that this strain is genetically closest to reference strains derived from parrots,suggesting potential host adaptation.Conclusion A relatively high detection rate of Cps was observed in samples collected from the parrot retail stall in a wholesale agricultural market in Shenzhen.It is recommended to strengthen hygiene management in bird and flower markets,especially at parrot stalls,and to enhance ongoing surveillance of Cps.关键词
鹦鹉热衣原体/基因组学/细胞分离/带菌情况调查分析Key words
Chlamydia psittaci/Genomics/Cell isolation/Carrier status investigation and analysis分类
医药卫生引用本文复制引用
崔文凯,吴双,陈琼城,雷欣悦,袁力,蔡健光,石晓路..深圳市某花鸟市场鹦鹉热衣原体采样调查与分离株基因组学研究[J].新发传染病电子杂志,2025,10(3):1-5,5.基金项目
1.广东省新发传染病应对病原体检测重点实验室(2023B1212010010) （2023B1212010010）
2.广东省自然科学基金一般研究项目(2514050002210) （2514050002210）
3.深圳市"医疗卫生三名工程"项目(SZSM202211023) （SZSM202211023）
4.深圳市自然科学基金基础研究项目(JCYJ20240813160806009) （JCYJ20240813160806009）
