右江医学2025，Vol.53Issue(7)：583-591,9.DOI:10.3969/j.issn.1003-1383.2025.07.002
基于网络药理学和分子对接的葛根素治疗视网膜静脉阻塞作用机制的研究
A study on the mechanism of puerarin in the treatment of retinal vein occlusion based on network pharmacology and molecular docking
摘要
Abstract
Objective To study the key targets and mechanisms of puerarin in the treatment of retinal vein occlusion(RVO)based on network pharmacology and molecular docking technology.Methods The chemical structure information of puerarin was adopted,and then the targets of puerarin were searched through SwissTargetPrediction database and SuperPred database,and RVO-related targets were obtained through GeneCards database and OMIM database.The intersection between the targets of puerarin and the RVO-related targets were taken,and then the potential targets of puerarin for treating RVO were obtained.Protein-protein interaction(PPI)analysis was conducted on the STRING platform,and the results were visu-alized and calculated by Cytoscape 3.10.3 software,so as to obtain the potential key targets of puerarin for treating RVO.Gene ontology(GO)functional enrichment and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis were performed with Metascape Version 3.5 to explore the potential mechanisms by which puerarin acted against retinal vein occlu-sion(RVO).In addition,molecular docking technology were employed to validate the binding affinities between puerarin and its key targets.Results After screening,130 potential targets for puerarin in the treatment of RVO were identified,including 20 potential key targets.GO functional enrichment analysis showed that these targets were mainly related to biologi-cal processes such as oxidative stress response and cell migration,cellular components such as extracellular matrix and plas-ma membrane lipid raft,kinase binding,oxidoreductase activity and other molecular functions.KEGG signaling pathway enrichment analysis showed that the main pathways of puerarin in the treatment of RVO were HIF-1 signaling pathway,MAPK pathway and AGE-RAGE signaling pathway in diabetic complications.Molecular docking showed that puerarin had strong binding activity with six targets.Conclusion Puerarin may act on targets such as PTGS2,AKT1,MAPK3,CASP3,TNF,and IL-6,and play a role in the treatment of RVO by regulating HIF-1,MAPK,AGE-RAGE signaling pathway in dia-betic complications and other signaling pathways.关键词
网络药理学/葛根素/视网膜静脉阻塞/分子对接Key words
network pharmacology/puerarin/retinal vein occlusion(RVO)/molecular docking分类
医药卫生引用本文复制引用
郑俊,刘靖,李小珍,潘洪平..基于网络药理学和分子对接的葛根素治疗视网膜静脉阻塞作用机制的研究[J].右江医学,2025,53(7):583-591,9.基金项目
广西自然科学基金项目(2023GXNSFBA026187) （2023GXNSFBA026187）
