中国比较医学杂志2025，Vol.35Issue(7)：44-54,11.DOI:10.3969/j.issn.1671-7856.2025.07.005
鳖甲煎丸对肝癌Huh7细胞恶性生物学行为的影响及机制研究
Study on the effect and mechanism of Biejiajian pill on the malignant biological behaviors of hepatocellular carcinoma Huh7 cells
摘要
Abstract
Objective To elucidate the effects and mechanisms of Biejiajian pill(BJJP)-containing serum on the malignant biological behaviors of hepatocellular carcinoma Huh7 cells.Methods This research knocked down CKLF-like MARVEL transmembrane domain containing 6(CMTM6)expression using a CMTM6-specific small interfering RNA(siRNA).Healthy Sprague-Dawley rats were used to prepare normal rat serum and low-(0.55 g/kg),medium-(1.1 g/kg),and high-(2.2 g/kg)BJJP-containing.Huh7 cells were cultured with normal fetal bovine serum(BC),normal rat serum(NC),and low-,medium-,and high-dose BJJP serum(LBJJP,MBJJP,and HBJJP,respectively).BJJP-containing serum and si-CMTM6 were applied to Huh7 cancer cells,and the proliferation,migration,and invasion abilities were evaluated by CCK-8 and Transwell assays,respectively.Protein expression levels of proliferating cell nuclear antigen(PCNA),epithelial-mesenchymal transition(EMT)markers,and CMTM6 were detected by Western blot.Results CMTM6 knockdown significantly reduced the mRNA and protein expression level of CMTM6 in Huh7 cells(P＜0.05).There were no significant differences between the BC and NC groups in terms of cell proliferation,migration,invasion,expression levels of PCNA,EMT markers,and CMTM6(all P＞0.05).BJJP-containing serum markedly inhibited Huh7 cell proliferation,migration,and invasion(P＜0.05),downregulated PCNA,CMTM6,N-cadherin,and Vimentin expression,and upregulated E-cadherin compared with the NC group(all P＜0.05).CMTM6 knockdown suppressed malignant behaviors,with reduced PCNA,Vimentin,and N-cadherin and elevated E-cadherin expression(all P＜0.05).Conclusions BJJP-containing serum can significantly inhibit Huh7 cell growth,invasion,migration,and EMT progression,potentially mediated via CMTM6 suppression.关键词
鳖甲煎丸/肝癌/增殖/迁移/侵袭/上皮-间充质转化/CMTM6Key words
Biejiajian pill/hepatocellular carcinoma/proliferation/migration/invasion/epithelial-mesenchymal transition/CMTM6分类
医药卫生引用本文复制引用
李以恒,徐隽婕,兰涛,李鑫,张荣花,熊亚南,朱丽华,章广玲..鳖甲煎丸对肝癌Huh7细胞恶性生物学行为的影响及机制研究[J].中国比较医学杂志,2025,35(7):44-54,11.基金项目
河北省自然科学基金资助项目(H2023209047,H2024209077) （H2023209047,H2024209077）
河北省中央引导地方科技发展资金项目(246Z7720G) （246Z7720G）
河北省创新能力提升计划——高水平人才团队建设专项(205A7701H). （205A7701H）
