中国动物传染病学报2025，Vol.33Issue(3)：1-9,9.
鸡白痢沙门氏菌yihE基因缺失株的构建及其相关生物学特性
Construction and Biological Characterization of a Salmonella Pullorum yihE Gene Deletion Strain
摘要
Abstract
To investigate the role of Salmonella Pullorum(ATCC19945)(WT,the same below)myceline-threonine protein kinase(YihE)in the pathogenic process,In this study,a gene deletion strain(ATCC19945ΔyihE)(KO,the same below)and a gene complementing strain(ATCC19945ΔyihE/pBR322-yihE)(RS,the same below)were constructed by λ-Red homologous recombination technology in the present study.Growth characteristics,anti-stress,locomotion,biofilm formation,drug resistance,anti-egg killing,bacterial adhesion,invasion and survival were determined for WT,KO and RS strains.The results showed that compared with the WT and RS strains,the KO strains had no significant difference in growth rate,locomotion and anti-egg killing while the anti-stress ability decreased by about 30%(except for heat stress of 42℃)the biofilm formation by about 40%.The formation ability of frillin as the main component of the biofilm,decreased while the visual appearance of cellulose as the main component of the biofilm did not change significantly.In addition,the sensitivity to quinolones was enhanced while adhesion,invasion and survival ability decreased.These results showed that the yihE gene was involved in multiple biological processes of Salmonella Pullorum and deletion of the yihE gene reduced relevant biological activities of Salmonella pullorum,which laid the foundation for further research on the pathogenic mechanism.关键词
鸡白痢沙门氏菌/yihE/抗应激能力/生物被膜Key words
Salmonella Pullorum/yihE/stress resistance/biofilm分类
农业科技引用本文复制引用
刘畅,张志强,张雪佳,王利丽,白和平,王苗,李浩,刘勃兴,史秋梅,吴同垒..鸡白痢沙门氏菌yihE基因缺失株的构建及其相关生物学特性[J].中国动物传染病学报,2025,33(3):1-9,9.基金项目
国家自然科学基金项目(31802186) （31802186）
河北省自然科学基金项目(C2019407070) （C2019407070）
河北省高等学校科学技术研究项目(BJ2021055) （BJ2021055）
河北省现代农业产业体系岗位专家项目 （）
