中国动物传染病学报2025，Vol.33Issue(3)：36-41,6.
乙肝核心抗原嵌合PEDV S1抗原表位病毒样颗粒疫苗的免疫原性评价
Construction and Immunogenicity of Hepatitis B Core Antigen Virus-Like Particles Carrying PEDV S1 Epitope
摘要
Abstract
In order to construct hepatitis B core antigen virus-like particles(VLPs)carrying PEDV S1 epitope and evaluate their immunogenicity in this study,the DNA sequence of the PEDV spike protein B-cell epitope 748YSNIGVCK755 was synthesized and cloned into the HBcAg backbone of major immunodominant region(MIR)and substitutions on a pET-28a(+)plasmid,then transformed into the BL21(DE3).The bacterial culture was grown overnight with IPTG for protein expression.The recombinant protein was purified and confirmed in SDS-PAGE and Western blot and VLPs were observed under transmission electron microscopy.The results showed that pET-28a(+)-HBcAg-PEDV S1 was constructed and the recombinant protein was expressed as inclusion bodies.The purified and renatured recombinant protein assembled as VLPs.Then,the recombinant protein was used to immunize BALB/c mice to evaluate its immunogenicity.High level of IFA antibodies and neutralizing antibodies were detected in the immunized mice.In summary,the recombinant protein HBcAg-PEDV S1 was expressed as VLPs and showed high immunogenicity in mice,which might be developed as a novel PEDV vaccine.关键词
猪流行性腹泻/乙肝核心抗原/病毒样颗粒/免疫原性Key words
Porcine epidemic diarrhea virus/hepatitis B core antigen/virus-like particles/immunogenicity分类
农业科技引用本文复制引用
赵淑庆,李基棕,李彬,张宝太,刘孟冉,薛涛,钟纯燕,周金柱,刘传敏,主性,倪艳秀..乙肝核心抗原嵌合PEDV S1抗原表位病毒样颗粒疫苗的免疫原性评价[J].中国动物传染病学报,2025,33(3):36-41,6.基金项目
十四五国家重点研发计划项目(2021YFD1800014) （2021YFD1800014）
江苏省农业科技自主创新资金项目(CX(20)3094)国家自然科学基金(31802167、31872481和31941013) （CX(20）
江苏省自然科学基金(BK20190003,BK20191235) （BK20190003,BK20191235）
山东省自然科学基金(ZR2020MC019) （ZR2020MC019）
