中国动物传染病学报2025，Vol.33Issue(3)：51-55,5.
猪德尔塔冠状病毒昆明小鼠感染模型的建立
Establishment of Kunming Mouse Model of Porcine Deltacoronavirus Infection
摘要
Abstract
To establish a Kunming mouse model of porcine deltacoronavirus(PDCoV),8-week-old SPF female Kunming mice were inoculated with 107.6 TCID50/mL of PDCoV JS2211 strain in this study.The infected group was given 200 uL PDCoV by gavage needle and 100 μL PDCoV by intramuscular injection.The control group was given equal volume of normal saline in the same way as the infection group.The clinical signs of mice were observed every day.At 1,3,5,7,10,14 and 21 days post infection,3 mice in the infection group and 1 mouse in the control group were randomly sacrificed to collect organs for histopathological observation and virus distribution by qRT-PCR.The results showed that the infected mice developed intestinal inflating,edema,wall thinning,mesenteric bleeding and other gastrointestinal lesions on 7 d,14 d and 21 d post infection.The histopathological observations found intestinal villi were partially defected with low density,shortness,thinner walls.Additionally,obvious bleeding in the mesentery,superficial gastric mucosal epithelial cells were accompanied by a little erosion and ablative.Many inflammatory cells were seen in these tissues.qRT-PCR showed that a large number of viruses were detected in the intestine tissues of infected mice and the level of viruses peaked at 14 days post infection.In conclusion,this study provided a useful animal model for the evaluation of antiviral biological products and drugs for PDCoV infection.关键词
猪德尔塔冠状病毒/昆明小鼠/动物模型Key words
Porcine deltacoronavirus/Kunming mouse/animal model分类
农业科技引用本文复制引用
宋俊菡,王元红,邓小莺,王俊娜,虞凌雪,王斌,周艳君,童光志,李国新..猪德尔塔冠状病毒昆明小鼠感染模型的建立[J].中国动物传染病学报,2025,33(3):51-55,5.基金项目
"十四五"国家重点研发计划课题(2022YFD1800805) （2022YFD1800805）
