中国现代医学杂志2025，Vol.35Issue(16)：28-34,7.DOI:10.3969/j.issn.1005-8982.2025.16.005
LncRNA CD36-005对大鼠子宫内膜蜕膜化的作用机制研究
Investigation of the mechanism by which lncRNA CD36-005 regulates decidualization in the rat endometrium
摘要
Abstract
Objective To explore the association between lncRNA CD36-005 and endometrial decidualization,as well as the role of lncRNA CD36-005 in decidualization of the rat endometrium.Methods Models of early pregnancy and artificially induced decidualization in rat uteri were established.The expression level of lncRNA CD36-005 was detected by quantitative real-time polymerase chain reaction(qRT-PCR).An in vitro model of decidualization was established using endometrial stromal cells.Following overexpression or knockdown of lncRNA CD36-005,the expression levels of decidualization marker genes were assessed by qRT-PCR.Results The relative expression level of lncRNA CD36-005 in the uterine tissues of female rats on gestation days 5-8 was significantly lower than that on days 1-4(P＜0.05).On gestation days 6-8,the expression of lncRNA CD36-005 at implantation sites was significantly lower than that at non-implantation sites(P＜0.05).In both artificially induced decidualized uterine tissues and decidualized endometrial stromal cells,lncRNA CD3 6-005 expression was markedly reduced(P＜0.05).Overexpression of lncRNA CD36-005 in decidualized stromal cells significantly inhibited the expression of Hand2 and Prl8a2(P＜0.05),whereas knockdown of lncRNA CD36-005 significantly increased the expression of these two genes(P＜0.05).Conclusion LncRNA CD36-005 is lowly expressed in the uterus during early pregnancy,in artificially induced decidualized uteri,and in decidualized endometrial stromal cells of rats,and it exerts an inhibitory effect on endometrial decidualization.关键词
子宫/基质细胞/蜕膜化/长链非编码RNA/lncRNA CD36-005Key words
uterus/stromal cell/decidualization/long non-coding RNA/lncRNA CD36-005分类
医药卫生引用本文复制引用
张雪莹,徐影,付璐璐,张京顺,王敏,蔡敏,郑连文..LncRNA CD36-005对大鼠子宫内膜蜕膜化的作用机制研究[J].中国现代医学杂志,2025,35(16):28-34,7.基金项目
吉林省科技厅科技发展计划项目(No:YDZJ202201ZYTS060) （No:YDZJ202201ZYTS060）
