中国药房2025，Vol.36Issue(15)：1876-1881,6.DOI:10.6039/j.issn.1001-0408.2025.15.10
健脾和胃方的指纹图谱、化学模式识别分析及多指标含量测定
Fingerprints,chemical pattern recognition analysis,and multi-index content determination of Jianpi hewei formula
摘要
Abstract
OBJECTIVE To establish HPLC fingerprint for Jianpi hewei formula(JPHWF),conduct chemical pattern recognition analysis,and determine the contents of seven components in the formula,aiming to provide a scientific basis for quality control and further research of JPHWF.METHODS Taking 15 batches of standard decoctions of JPHWF as samples,the HPLC fingerprint was established using the Similarity Evaluation System of TCM Chromatographic Fingerprint(2012 edition).Subsequently,similarity evaluation,as well as identification and attribution analysis of chromatographic peaks,were conducted.Using the common peak areas from the 15 batches of samples as variables,chemical pattern recognition analyses were performed on the samples through hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis.The contents of adenine,5-hydroxymethylfurfural,tetrahydropalmatine,naringin,dehydrocorydaline,neohesperidin and glycyrrhizic acid in 15 batches of samples were determined by HPLC.RESULTS There were 19 common peaks in the characteristic chromatograms for 15 batches of samples with the similarities of more than 0.95.Results of chemical pattern recognition analysis showed that 15 batches of samples could be clustered into 3 categories,and 3 differential compounds were found[peak 7(5-hydroxymethylfurfural),peak 17(neohesperidin),and peak 15(naringin)].The 7 components were linearly good in the respective concentration ranges(R2≥0.999 4);RSDs of precision,stability and repeatability tests were less than 2%(n=6);the average recovery rate of 98.95%-103.81%,RSD of 0.61%-2.75%(n=6);the contents of them were 0.031-0.106,0.267-0.824,0.089-0.144,1.344-2.091,0.089-0.178,1.328-2.028,0.040-0.150 mg/g,respectively.CONCLUSIONS Established HPLC fingerprinting method coupled with multi-index content determination is validated to be accurate and reliable,and its combination with chemical pattern recognition analysis can be applied to the quality control of JPHWF.关键词
健脾和胃方/指纹图谱/化学成分/含量测定/层次聚类分析/主成分分析/正交偏最小二乘-判别分析Key words
Jianpi hewei formula/fingerprint/chemical constituents/content determination/hierarchical cluster analysis/principal component analysis/orthogonal partial least squares-discriminant analysis分类
医药卫生引用本文复制引用
何东栋,宗慧,王重阳,宛娟娟,毛学璞,黄川生,王新春,王立萍..健脾和胃方的指纹图谱、化学模式识别分析及多指标含量测定[J].中国药房,2025,36(15):1876-1881,6.基金项目
兵团科技计划项目重点领域科技攻关项目(No.2023AB045,No.2024AB071) （No.2023AB045,No.2024AB071）
兵团指导性科技计划(No.2022ZD048) （No.2022ZD048）
石河子大学高层次人才科研启动专项(No.RCZK202374) （No.RCZK202374）
