中国医学工程2025，Vol.33Issue(7)：7-10,4.DOI:10.19338/j.issn.1672-2019.2025.07.002
关于解决磁微粒化学发光试剂稳定性问题的研究
Stability of chemiluminescence reagent of magnetic particle
豆娅茹 1李忠信1
作者信息
- 1. 郑州安图生物工程股份有限公司,研发中心免疫学部,河南 郑州 450016
- 折叠
摘要
Abstract
[Objective]The stability of magnetic particle chemiluminescence products is an important property of magnetic particle reagents,and most items are unstable at 37℃.In this paper,the method of improving the stability of magnetic particle reagent was discussed by taking the anti-SS-A/Ro antibody IgG detection kit(magnetic particle chemiluminescence method)as an example.[Methods]The stability of the reagent was improved by adjusting the pH of the buffer,using different protein sealers and increasing the concentration of salt ions in the buffer.[Results]Increasing the concentration of salt ions in the buffer can effectively reduce the non-specific adsorption and improve the stability of the reagent.[Conclusion]There are many kinds of coated antigens in magnetic particle reagents,and the activity of antigens varies depending on the extraction method.After selecting the appropriate coated antigen,it is necessary to analyze the physicochemical properties and extraction methods of the coated antigen used and the characteristics of the antigen itself.Thus,the magnetic particle coating method can be better selected to improve the stability of the reagent,reduce the non-specific adsorption,and improve the performance of the reagent.关键词
自身免疫/非特异性吸附/磁微粒化学发光Key words
autoimmune disease/nonspecific adsorption/magnetic particles chemiluminescence分类
医药卫生引用本文复制引用
豆娅茹,李忠信..关于解决磁微粒化学发光试剂稳定性问题的研究[J].中国医学工程,2025,33(7):7-10,4.
