中国药业2025，Vol.34Issue(15)：68-72,5.DOI:10.3969/j.issn.1006-4931.2025.15.012
二甲双胍增敏吉西他滨对人胰腺癌细胞的抑制作用及其机制
Metformin Sensitizing the Inhibitory Effect of Gemcitabine on Human Pancreatic Cancer Cells and Its Mechanism
摘要
Abstract
Objective To investigate the mechanism of metformin in enhancing the sensitivity of the inhibitory effect of gemcitabine on human pancreatic cancer cells(aspc-1).Methods Cells were divided into the blank control group(group A,conventional culture medium),metformin group(group B,conventional culture medium with 5.00 mmol/L metformin),gemcitabine group(group C,conventional culture medium with 2.50 μg/L gemcitabine),and combined group(group D,conventional culture medium with 5.00 mmol/L metformin+2.50 μg/L gemcitabine).After treatment,cells in each group were cultured for 48 h.Cell apoptosis was detected by the flow cytometry,and the expression levels of P-glycoprotein(P-gp),hypoxia inducible factor-1α(HIF-1α),and phos phorylatod nuclear factor κB(p-NF-κB)proteins were detected by the Western blot.Results Compared with those in group A,the apoptosis rates of cells in groups C and D significantly increased(P<0.05).Compared with those in group C,the apoptosis rate of cells in group D significantly increased(P<0.05).Compared with those in group A,the expression levels of P-gp,HIF-1α,and p-NF-κB p65 proteins in group B significantly decreased(P<0.05),while the above indexes in group C significantly increased(P<0.05);compared with those in group C,the above indexes in group D significantly decreased(P<0.05).Conclusion Metformin can enhance the inhibitory effect of gemcitabine on human pancreatic cancer cell aspc-1,and its mechanism may be related to the down-regulation of P-gp to enhance the sensitivity of aspc-1 to gemcitabine.关键词
二甲双胍/吉西他滨/胰腺癌细胞/P糖蛋白/缺氧诱导因子-1α/作用机制Key words
metformin/gemcitabine/pancreatic cancer cells/P-glycoprotein/hypoxia inducible factor-1α/mechanism分类
医药卫生引用本文复制引用
张晓丹,李光慧,袁媛,王利苹,黄仲义,王斌..二甲双胍增敏吉西他滨对人胰腺癌细胞的抑制作用及其机制[J].中国药业,2025,34(15):68-72,5.基金项目
上海市临床药学重点专科建设项目[沪卫计药政[2018]9号] （）
上海市静安区医学科研课题[2017MS03] （）
上海市静安区医学重点学科建设项目[2024ZD01]. （）
