中国医院用药评价与分析2025，Vol.25Issue(7)：816-822,7.DOI:10.14009/j.issn.1672-2124.2025.07.010
基于成分-靶点-通路网络联合GEO数据库比较调冲方与来曲唑治疗多囊卵巢综合征的潜在分子作用机制
Comparison on Potential Molecular Mechanism of Tiaochong Decoction and Letrozole in the Treatment of Polycystic Ovary Syndrome Based on Component-Target-Pathway Combined with GEO Database
摘要
Abstract
OBJECTIVE:To probe into the mechanism of Tiaoxiong decoction by Professor Xu Runsan combined with letrozole in the treatment of polycystic ovary syndrome(PCOS)based on the chip mining technology from the GEO database,network pharmacology and molecular docking technology.METHODS:The active components of Tiaoxiong decoction were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.The Swiss Target Prediction database was searched to obtain the drug-related targets of Tiaochong decoction and letrozole.Differential gene analysis was performed on the GEO database,the targets related to PCOS were obtained from OMIM,GeneCards,DisGeNET,and CTD databases,and the network diagram of drug-component-target was drawn by using Cytoscape 3.8.0 software.The protein-protein interaction network was constructed by STRING database and Cytoscape 3.8.0,and the key targets were screened.Gene ontology(GO)functional enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were performed based on the DAVID platform.The molecular docking of key active components and core targets was verified by using AutoDock Tools.RESULTS:There were 176 active components and 467 unique targets of Tiaochong decoction;there were 69 unique targets of letrozole,with 54 common targets of both.Enrichment analysis revealed that the treatment group had 1 775 GO entries and 190 KEGG pathways;letrozole had 344 GO entries and 123 KEGG pathways;and both groups shared 309 GO entries and 123 KEGG pathways.Molecular docking results showed that the key active components of Tiaochong decoction,letrozole and the core targets all had better binding activities.CONCLUSIONS:There are both commonalities and differences in the targets and pathways of Tiaochong decoction and letrozole in the treatment of PCOS,which can control the occurrence and development of PCOS through their own unique targets.关键词
调冲方/来曲唑/多囊卵巢综合征/网络药理学/分子对接Key words
Tiaochong decoction/Letrozole/Polycystic ovary syndrome/Network pharmacology/Molecular docking分类
医药卫生引用本文复制引用
李娇月,马小娜,沈希,梁海燕..基于成分-靶点-通路网络联合GEO数据库比较调冲方与来曲唑治疗多囊卵巢综合征的潜在分子作用机制[J].中国医院用药评价与分析,2025,25(7):816-822,7.基金项目
中日友好医院高水平医院临床业务费专项(No.2022-NHLHCRF-LX-02-0117) （No.2022-NHLHCRF-LX-02-0117）
