中成药2025，Vol.47Issue(7)：2144-2148,5.DOI:10.3969/j.issn.1001-1528.2025.07.002
HPLC法同时测定补脑软胶囊中11种成分的含量
Simultaneous content determination of eleven constituents in Bunao Soft Capsules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of 5-hydroxymethylfurfural,chlorogenic acid,caffeic acid,strychnine,paeoniflorin,ferulic acid,paeoniflorin Ⅰ,epimedium glycoside,psoralen,isopsoralen and glycyrrhetinic acid in Bunao Soft Capsules.METHODS The analysis was performed on a 35 ℃ thermostatic Waters Symmetry C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,280 nm.RESULTS Eleven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 98.47％-103.30％with RSDs of 1.13％-2.80％.CONCLUSION This simple and reliable method can be used for the quality control of Bunao Soft Capsules.关键词
补脑软胶囊/化学成分/含量测定/HPLCKey words
Bunao Soft Capsules/chemical constituents/content determination/HPLC分类
医药卫生引用本文复制引用
陈挺,王雪梅,李帅印,李喜香,周亚丽,李小凤,杨寿圆..HPLC法同时测定补脑软胶囊中11种成分的含量[J].中成药,2025,47(7):2144-2148,5.基金项目
兰州市科技计划基金资助项目(2023-2-19) （2023-2-19）
