中成药2025，Vol.47Issue(7)：2163-2171,9.DOI:10.3969/j.issn.1001-1528.2025.07.005
基于谱效关系筛选大黄炭制前后改善人脐静脉内皮细胞损伤的物质基础
Screening of material basis for Rhei Radix et Rhizoma on improving human umbilical vein endothelial cell injury before and after carbonization based on spectrum-effect relationship
摘要
Abstract
AIM To screen the material basis for Rhei Radix et Rhizoma on improving human umbilical vein endothelial cell(HUVECs)injury before and after carbonization.METHODS The HPLC fingerprints were established,after which difference analysis was performed by orthogonal partial least squares discriminant analysis.The LPS-induced HUVECs injury model was established,then NO,MDA levels and SOD activity were detected.Gray correlation analysis and partial least squares regression were adopted in the investigation of spectrum-effect relationship,and active constituents were screened.RESULTS There were 22 and 20 common peaks in the fingerprints for 17 batches of raw products and carbonized products,respectively,along with the similarities of more than 0.8.Twelve main difference components were observable,among which gallic acid,5-hydroxymethylfurfural,catechin,aloe rhodopsin-8-O-β-D-glucoside,rhubarbic acid-8-O-β-D-glucoside and sennosides A were identified.The carbonized products demonstrated stronger effect on improving HUVECs injury than the raw product.The correlations of common peaks were more than 0.5 in the fingerprints for raw products and carbonized products,and peaks 3,5,11,12,15,19,23 exhibited significant effects on their efficacy(VIP values＞1).CONCLUSION This accurate,reliable and reproducible method can provide a basis for clarifying the material basis for hemostatic efficacy of Rhei Radix et Rhizoma and its carbonized product.关键词
大黄/大黄炭/人脐静脉内皮细胞(HUVECs)/物质基础/HPLC指纹图谱/止血作用/谱效关系Key words
Rhei Radix et Rhizoma/Rhei Radix et Rhizoma charcoal/human umbilical vein endothelial cells(HUVECs)/material basis/HPLC fingerprints/hemostatic efficacy/spectrum-effect relationship分类
医药卫生引用本文复制引用
翟婷,卢利涛,何江伟,郭亚菲,郭玫,朱昕宇..基于谱效关系筛选大黄炭制前后改善人脐静脉内皮细胞损伤的物质基础[J].中成药,2025,47(7):2163-2171,9.基金项目
国家自然科学基金联合基金重点项目(U21A20412) （U21A20412）
甘肃省教育厅高校教师创新基金项目(2024A-091) （2024A-091）
甘肃省道地药材质量标准化技术研究与推广工程实验室开放基金项目(ddyc-2022-04) （ddyc-2022-04）
甘肃中医药大学引进人才科研启动基金(2019YJRC-01) （2019YJRC-01）
