中成药2025，Vol.47Issue(7)：2171-2177,7.DOI:10.3969/j.issn.1001-1528.2025.07.006
白及提取工艺优化及其抗氧化、酪氨酸酶抑制活性评价
Optimization of extraction process for Bletillae Rhizoma and evaluation of its anti-oxidant,tyrosinase inhibitory activities
摘要
Abstract
AIM To optimize the extraction process for Bletillae Rhizoma,and to evaluate its anti-oxidant,tyrosinase inhibitory activities.METHODS With ultrasound time,ethanol concentration and solid-liquid ratio as influencing factors,the total extraction content of gastrodin,protocatechualdehyde,p-hydroxybenzaldehyde,1,4-bis[4-(gluconoxy)benzyl]-2-isobutylmalate-2-glucoside,1,4-bis[4-(gluconoxy)benzyl]-2-isobutylmalate,yam Ⅲ,dihydropinosin and 3'-O-methylyam Ⅲ as an evaluation index,the extraction process was optimized by Box-Behnken response surface method.Subsequently,the extract's scavenging effects on DPPH,ABTS+free radicals,and inhibitory ability on tyrosinase were determined.RESULTS The optimal conditions were determined to be 49 min for ultrasound time,55％for ethanol concentration,1∶30 for solid-liquid ratio,and 2 times for extraction frequency,the total extraction content was 13.18 mg/g.The extract demonstrated the IC50 of 10.12,314.07 and 1.70 μg/g on DPPH,ABTS+free radicals and tyrosinase,respectively.CONCLUSION This simple,reliable and stable method can be used for the extraction for Bletillae Rhizoma with strong anti-oxidant,tyrosinase inhibitory activities.关键词
白及/提取工艺/抗氧化活性/酪氨酸酶抑制活性/Box-Behnken响应面法Key words
Bletillae Rhizoma/extraction process/anti-oxidant activity/tyrosinase inhibitory activity/Box-Behnken response surface method分类
医药卫生引用本文复制引用
王珊珊,罗益远,谢叶菲,蒋新苗,潘萍,秦昆明,陈宏降..白及提取工艺优化及其抗氧化、酪氨酸酶抑制活性评价[J].中成药,2025,47(7):2171-2177,7.基金项目
浙江省公益基础研究项目(LTGN23H280004) （LTGN23H280004）
浙江省药品监督管理局科技计划项目(2023014) （2023014）
浙江省教育厅一般项目(Y202352650) （Y202352650）
宁波市自然科学基金项目(2021J187,2023J306) （2021J187,2023J306）
